Genotyping and primer details


Genotyping and primer details for 526H09 - At5g39740
Line ID 526H09
Locus At5g39740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:15904450 of line 526H09 Chr5:15904450
   
Insertion allele amplicon 8474 - 24kn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 24kn: 5'-GACATTGTTAAAGCTTCGGCTTAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:15904100
   
wt amplicon (over InsPos) 07le - 24kn
wt amplicon start Chr5:15904100
wt amplicon end Chr5:15905033
wt amplicon size 934 bp
Locus specific primer 24kn: 5'-GACATTGTTAAAGCTTCGGCTTAC-3'
2nd locus specific primer 07le: 5'-GAGACAAACCAATCACAACAAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11