Sequences from confirmation amplicon


Confirmation sequences for line 527F08 and hit At5g38390 are shown.
Line ID 527F08
Locus At5g38390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:15367457
Primers used for PCR JL75 - 8474, show primer details
   
Sequence Name 44-K101384-0022-527-F08C-8409
GenBank Accession LN505123 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K101384-0022-527-F08C-8409
ATAAGCAAGGTCTATTATGGTTTCTCTTGTTATGAGGTGCTTTCTCTATGCTGTGAATCG
ATGCCAGTGTTCAAAAACCTCAAATCTTTGTCTATCAAGAGTGCCGAGAATCGAGGATGG
CAAGCAATGCCAGTTCTTCTAAGGAACTGTCCGCATTTAGAAACTCTAGTCCTTGAGGTA
CGTATAACACAATTCATTAATTTGCCAAACATGTACAAAATATCATACTTTGAATTTCTC
ATTGTCACTTTTATGCTTTCAGGGTCTATTGCACCATGTAACAGATAAATGTGGGGATGC
TTGTGACTGTGTTTCCCGAGAAGACAAAGGTCGTTCACTCACATCGTGTCCAGTAAAAGT
GTTGGAGATTAAAGGGTTCCAAGGAACAACCAAAGAGATGAACATGATAAAACATTTCTG
GGAATATTTTCCGTGTTTGAA
   
Sequence Name 44-K101394-0022-527-F08C-JL75
GenBank Accession LN505124 [GenBank]
Sequencing primer gene specific primer, JL75: 5'-ATTTTTAGCTCTTTCAAACACGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K101394-0022-527-F08C-JL75
TGTTCTCTCTTTGGTTGTTCCTTGGAACCCTTTAATCTCCAACACTTTTACTGGACACGA
TGTGAGTGAACGACCTTTGTCTTCTCGGGAAACACAGTCACAAGCATCCCCACATTTATC
TGTTACATGGTGCAATAGACCCTGAAAGCATAAAAGTGACAATGAGAAATTCAAAGTATG
ATATTTTGTACATGTTTGGCAAATTAATGAATTGTGTTATACGTACCTCAAGGACTAGAG
TTTCTAAATGCGGACAGTTCCTTAGAAGAACTGGCATTGCTTGCCATCCTCGATTCTCGG
CACTCTTGATAGACAAAGATTTGAGGTTTTTGAACACTGGCATCGATTCACAGCATAGAG
AAAGCACCTCATAACAAGAGAAACCATAATTAGACCTTTGCTTATTATATTTGTTAAAAA
TGGGACCAGCAATGAGTATGATGGTCAATATGGAGAANAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCANAAATGTAGATNTCCGC

Last Updated on 06.12.2018 16:11