Sequences from confirmation amplicon


Confirmation sequences for line 527H04 and hit At3g62270 are shown.
Line ID 527H04
Locus At3g62270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23043260
Primers used for PCR TF10 - 8474, show primer details
   
Sequence Name 35-K027640-022-527-H04C-8409
GenBank Accession LN501696 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K027640-022-527-H04C-8409
GCCATTTACATTGAATAATTCTTTCCCAGAATTGGTTTCCGGGTAAGCTTTCGATTGCCA
TGAAGGCAAAGTAGCCCCAAAGGACTGATGTAGGGATCATTTTAAGGAGAGGCATAGCTG
CAACACACCCTCCAACCATTACTGCTTGAAGCAAGTTGCTTACTCTCTGTTCTTTGACTT
CAATTGGCAATAAATCATCAATCTCTTTCTCGATATCAAACAGAGTCTCATCAACTGGAG
CATCGAGATTTCCTGTGAATGTTGTTGCTTGGATTGTTGATTCTCTTAGCTCTTTCAGAC
CCTACAGAAGAGATAGTTTATCCACCACTTAGCCCCAAACCAAATTACTTTCCATTGAAA
AGTTACACAGGATATTCTTTACCTGAGGCTGTTGGTAGACTAATGGAGTCTGCATTTGAT
TATAAACATCTTGCATGTTTCCATACAACTGTCCTAGACTCGCATTCTGCTTGATGCTTT
TGCGAGCAGTTGCAACTAGTCTGTTCCGAAGCAGCTGTGGAAAGAGCAAACAAGAAGCT
   
Sequence Name 36-K027640-022-527-H04C-TF10
GenBank Accession LN502088 [GenBank]
Sequencing primer gene specific primer, TF10: 5'-CCTTGGATTTAAGCTTCTTGTTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K027640-022-527-H04C-TF10
TTGCAACTGCTCGCANAAGCATCAAGCAGAATGCGAGTCTAGGACAGTTGTATGGAAACA
TGCAAGATGTTTATAATCAAATGCAGACTCCATTAGTCTACCAACAGCCTCAGGTAAAGA
ATATCCTGTGTAACTTTTCAATGGAAAGTAATTTGGTTTGGGGCTAAGTGGTGGATAAAC
TATCTCTTCTGTAGGGTCTGAAAGAGCTAAGAGAATCAACAATCCAAGCAACAACATTCA
CAGGAAATCTCGATGCTCCAGTTGATGAGACTCTGTTTGATATCGAGAAAGAGATTGATG
ATTTATTGCCAATTGAAGTCAAAGAACAGAGAGTAAGCAACTTGCTTCAAGCAGTAATGG
TTGGAGGGTGTGTTGCAGCTATGCCTCTCCTTAAAATGATCCCTACATCAGTCCTTTGGG
GCTACTTTGCCTTCATGGCAATCGAAAGCTTACCCGGAAACCAATTCTGGGAAAGAATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGT
CGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTT

Last Updated on 06.12.2018 16:11