Sequences from confirmation amplicon


Confirmation sequences for line 528B11 and hit At4g17350 are shown.
Line ID 528B11
Locus At4g17350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9702163
Primers used for PCR CD75 - 8474, show primer details
   
Sequence Name 23-K105918-0022-528-B11C-CD75
GenBank Accession LN496556 [GenBank]
Sequencing primer gene specific primer, CD75: 5'-TGCATATCAACGAAACTGAAAAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K105918-0022-528-B11C-CD75
CACAAGCCTTACAGTTGCAGCAGCGGCTGTTAGAGTCATGAGATCACCGGGGCTGTGGAT
ATCCACAGAAGATCTAACCGTGGAAGTAACACGTGCATGGTCAGCACCGGCTCGCTCAGC
CATCTCAACACAGTGTGAGGCCAACAGTTCAGTGGCTGAGGCTAAAGCCAGTGCCATCTT
GGAGCCTGACCCTTTGCAATTGCTAGCAGATGTCACAGAGGCTAAACCTGCAGCCAGAGC
CGCTATAGAGACGGCAGAATGCACGTGGGCGTTCTCCACACGCACTTTATCTTTCTTCTT
GAGATTGGTAGTAGTACTGCTGGTGCTGTGCCAACAGACTATATTCAATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 23-K105919-0022-528-B11C-8409
GenBank Accession LN496557 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K105919-0022-528-B11C-8409
GCCATTTACAATTGAATATAGTCTGTTGGCACAGCACCAGCAGTACTACTACCAATCTCA
AGAAGAAAGATAAAGTGCGTGTGGAGAACGCCCACGTGCATTCTGCCGTCTCTATAGCGG
CTCTGGCTGCAGGTTTAGCCTCTGTGACATCTGCTAGCAATTGCAAAGGGTCAGGCTCCA
AGATGGCACTGGCTTTAGCCTCAGCCACTGAACTGTTGGCCTCACACTGTGTTGAGATGG
CTGAGCGAGCCGGTGCTGACCATGCACGTGTTACTTCCACGGTTAGATCTTCTGTGGATA
TCCACAGCCCCGGTGATCTCATGACTCTAACAGCCGCTGCTGCAACTGGTAAGGCTTTGT
TGCAGCTTCACAAGGCTTTTTCAGTTTCTTGAATATGC

Last Updated on 06.12.2018 16:11