Sequences from confirmation amplicon


Confirmation sequences for line 529B11 and hit At1g16520 are shown.
Line ID 529B11
Locus At1g16520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:5650823
Primers used for PCR 02de - 8474, show primer details
   
Sequence Name 23-K109936-0022-529-B11C-02de
GenBank Accession LN496598 [GenBank]
Sequencing primer gene specific primer, 02de: 5'-CAGCTTCAAAATACACTGGAAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K109936-0022-529-B11C-02de
CCGCAACACCAAAGATCATCTCCACAAGTGTGTCTCCTCGAGGCTATTCTGCAGCAGGTT
CTCCTAAAAGAACATCAGGTGCAGTTTCTCCCACAAAGGCAACACTCTGGTACCCGTCAA
GTCAGCAATCATCGGCTGCGAATTCTCCTCCTCGCAACCGCACACTCCCAGGTTCATAGG
ATCTTAAAGATTGTTACTTGTATAAAATTGGTAAAACTCAAGTATATTTTCTCATGTATA
AATTCTTTTCCAGCTCGTACACCGCGGATGGATGGTAAAGAATTCTTCCGGCAAGCCAGG
TGAGTATTTATTTCTTCAGTTCTTGTCTTAGCTAGTGTCGGTTTTTCATTAACCAGAATC
TGAAATTGTTGATCTTTTTCTGTTTCATTTGTAATTCACAGGAGCCGATTATCCTATGAA
CAATTCAGTTCCTTCTTAGCTAACATCAAAGAGCTCAACGCCCAGAAGCAAACCCGAGAG
GTAAATCCGGATCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 07-K109935-0022-529-B11C-8409
GenBank Accession LN489319 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K109935-0022-529-B11C-8409
CATTTACAATTGAATATATCCTGATCCGGATTTCCTCTCGGGTTTGCTTCTGGGCGTTGA
GCTCTTTGATGTTAGCTAAGAAGGAACTGAATTGTTCATAGGATAATCGGCTCCTGTGAA
TTACAAATGAAACAGAAAAAGATCAACAATTTCAGATTCTGGTTAATGAAAAACCGACAC
TAGCTAAGACAAGAACTGAAGAAATAAATACTCACCTGGCTTGCCGGAAGAATTCTTTAC
CATCCATCCGCGGTGTACGAGCTGGAAAAGAATTTATACATGAGAAAATATACTTGAGTT
TTACCAATTTTATACAAGTAACAATCTTTAAGATCCTATGAACCTGGGAGTGTGCGGTTG
CGAGGAGGAGAATTCGCAGCCGATGATTGCTGACTTGACGGGTACCAGAGTGTTGCCTTT
GTGGGAGAAACTGCACCTGATGTTCTTTTAGGAGAACCTGCTGCAGAATAGCCTCGAGGA
GACACACTTGTGGAGATGATCTTTGGTGTTGCGGTTGGGGTAAGACGTGGAGATATGTAT
GATGTCATTGAAAATTTGTTTCCAGTGTATTTTGAAGC

Last Updated on 06.12.2018 16:11