Sequences from confirmation amplicon


Confirmation sequences for line 529D07 and hit At3g12520 are shown.
Line ID 529D07
Locus At3g12520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3971771
Primers used for PCR 23ke - 8474, show primer details
   
Sequence Name 84-K113595-0022-529-D07C-23ke
GenBank Accession LN514457 [GenBank]
Sequencing primer gene specific primer, 23ke: 5'-CACAATTTACTGGAGAGCACAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K113595-0022-529-D07C-23ke
TTTATGTGACAATCTAAGTATGAAAAATGATTGAATTTTAAAACTGAAAGAATGATGAAC
CTGAATCATGACAGGATATTTAGAGATGGTACTGTTCAAGTCAAGGCTTATACTTAACAG
TAATCAACGAAACATACTGATTAATTAAGACACGCAATGATATGATTAATGATCAAGATA
AAGTTATGAATGTAGCTGCCATATCGTCCTTATCCATCCCAAACTGGCACTACGCTATGC
TCTGTTTCTTCATGCTACAATCAGTGATACGTCTTTCTTCACGTTGATCACATTCTTCTC
TATCCCACCAATTCCGATGTCCCTCGCCGTCAAGGATCTCTCCACCGCTTCATCCTCTTC
ATCTAAGGCGATTCCGGTCAAGATCATTCCTCTCCAGTATCCCGATTCCACCTCTTCTGA
TCCTCACTGTCAATCCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTCAATTCAAAAATGTAGATGTCCGCACGGTT
   
Sequence Name 42-K113594-0022-529-D07C-8409
GenBank Accession LN504532 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K113594-0022-529-D07C-8409
ATCCTGGATTGACAGTGAGGATCAGAAGAGGTGGAATCGGGATACTGGAGAGGAATGATC
TTGACCGGAATCGCCTTAGATGAAGAGGATGAAGCGGTGGAGAGATCCTTGACGGCGAGG
GACATCGGAATTGGTGGGATAGAGAAGAATGTGATCAACGTGAAGAAAGACGTATCACTG
ATTGTAGCATGAAGAAACAGAGCATAGCGTAGTGCCAGTTTGGGATGGATAAGGACGATA
TGGCAGCTACATTCATAACTTTATCTTGATCATTAATCATATCATTGCGTGTCTTAATTA
ATCAGTATGTTTCGTTGATTACTGTTAAGTATAAGCCTTGACTTGAACAGTACCATCTCT
AAATATCCTGTCATGATTCAGGTTCATCATTCTTTCAGTTTTAAAATTCAATCATTTTTC
ATACTTAGATTGTCACATTAAACTAGCTGGTTTATGAGTTCGTATGTTTCAACCTTTTGT
GCTCTCCAGTAAATTGTG

Last Updated on 06.12.2018 16:11