Genotyping and primer details


Genotyping and primer details for 529D07 - At3g12520
Line ID 529D07
Locus At3g12520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3971771 of line 529D07 Chr3:3971771
   
Insertion allele amplicon 8474 - 23ke
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 23ke: 5'-CACAATTTACTGGAGAGCACAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3972264
   
wt amplicon (over InsPos) 01lb - 23ke
wt amplicon start Chr3:3971181
wt amplicon end Chr3:3972264
wt amplicon size 1084 bp
Locus specific primer 23ke: 5'-CACAATTTACTGGAGAGCACAAAA-3'
2nd locus specific primer 01lb: 5'-ACGAGAAGGGAAACTAATGCTACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11