Sequences from confirmation amplicon


Confirmation sequences for line 530G01 and hit At1g65850 are shown.
Line ID 530G01
Locus At1g65850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:24495642
Primers used for PCR JL69 - 8474, show primer details
   
Sequence Name 30-K101383-0022-530-G01C-8409
GenBank Accession LN499647 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K101383-0022-530-G01C-8409
CATTTACATTGAATATTCAGCATATTCGAAATATCAGTGGCAATGTTTTCAATCATGGTC
GCTTCATTATCCCTAATAAAGTTGGAAGATCAATAAATACATAATATAGTCATGAAAATT
TCAAGTACGTATTTAAAATATATAAATTTCTATATGGGAGATCATAAATATTTGTAGATA
CACATATATAATTTGTTGTGTTAAAAAGGATTAAGCCCGTATGTTCAGAAGGACGAACCA
GTTGCGAGAATCGTAACCAGTGGTTGTTCCCATTTTTGCCAGAGCTTGTCTCCATTTCCT
TATAACCTCATTAGTTTTACCAACACAATTATTTTTGAAGACATTCCCAAAATTTCCTGT
GAGCTTCTTAACATCGGATGGATCCACTCTATAGAANATGGGGATCACTATNTGACTAAN
TTCTTCTTTGCATTTCATGATCTCCACCAACTCGTCAAGACACCAAGATGAAGAAGCATA
GTTCCTAGAGAGCAAGACAATCGCGATCTTAGATTCTCTAATCGCAAGTATGAATTCCAG
AACAATGGATTCTCCTCTCTTGATCTCATTATCAATGAATGGTGTAATTCCCTTTCTTTG
AAACTCCTTGTGAATATGACTGAGAAAACCTCTACGTACATCTTCCCCACGGAAGCTTGG
AAAGACTTGATG
   
Sequence Name 30-K101389-0022-530-G01C-JL69
GenBank Accession LN499648 [GenBank]
Sequencing primer gene specific primer, JL69: 5'-CACAAGTGGACACATCAAGTCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K101389-0022-530-G01C-JL69
GGTTTTCTCAGTCATATTCACAGGAGTTTCAAAGAAAGGGAACTACTACCATTCATTGAT
AATGAGATCAAGAGAGGAGAATCCATTGGTCTTGAAATCATACATGCGATTAGAGAATCT
AAGATCGCGATTGTCTTGCTCTCTAGGAACTATGCTTCTTCATCTTGGTGTCTTGACGAG
TTGGTGGAGATCATGAAATGCAAAGAAGAATTTAGTCAAATAGTGATCCCCATTTTCTAT
AGAGTGGATCCATCCGATGTTAAGAAGCTCACAGGAAATTTTGGGAATGTCTTCAAAAAT
AATTGTGTTGGTAAAACTAATGAGGTTATAAGGAAATGGAGACAAGCTCTGGCAAAAATG
GGAACAACCACTGGTTACGATTCTCGCAACTGGTTCGTCCTTCTGAACATACGGGCTTAA
TCCTTTTTAACACAACAAATTATATATGTGTATCTACAAATATNTATGATCTCCCATATA
GAAATTTATATATNTTTAATACGTACTTGAAATTTTCATGACTATNTNATGTATTTATTG
ATCTTCCAACTTTATNAGGGATAATGAAGCGACCATGATTGAAA

Last Updated on 06.12.2018 16:11