Sequences from confirmation amplicon


Confirmation sequences for line 531F06 and hit At2g39400 are shown.
Line ID 531F06
Locus At2g39400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:16453734
Primers used for PCR 14wb - 8474, show primer details
   
Sequence Name 47-K118356-0022-531-F06C-14wb
GenBank Accession LN506333 [GenBank]
Sequencing primer gene specific primer, 14wb: 5'-ACTTGTTCTCCCTCACCTGTAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K118356-0022-531-F06C-14wb
CAAGCTGATATTACAAGTAAAATTTGGCGTCTTAGATCAGTATCACTCACCTGATTTCTG
ATGTGAGGTTCTTTAATTGCAATGTCAATAATATCATTTCCTGGAACTATTTTCCACGTT
GGAATAAACTTGGCTAACTTGATAAGGATTGAGATCACTACTGGATGTGGCTTTATCTCG
TCTGCTAACTGTCCATAACAACATTCATTGTATTAGTTGATAAACAAAACATTTTGATTA
TCTCTGTGGGACTTTCGGTAGTTGATATAATCATTTCGATAATTGAGTTTTGATTACTTG
TTTAATTAGTGGTATATATACTGGTATTTGGTTGTAACAAGTAGTTGTGTATATATAGAA
ACTATGAGACAAATGACTAATGAAGAGTAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 47-K118355-0022-531-F06C-8409
GenBank Accession LN506332 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K118355-0022-531-F06C-8409
TTACTCTTCATAGTCATTTGTCTCATAGTTTCTATATATACACAACTACTTGTTACAACC
AAATACCAGTATATATACCACTAATTAAACAAGTAATCAAAACTCAATTATCGAAATGAT
TATATCAACTACCGAAAGTCCCACAGAGATAATCAAAATGTTTTGTTTATCAACTAATAC
AATGAATGTTGTTATGGACAGTTAGCAGACGAGATAAAGCCACATCCAGTAGTGATCTCA
ATCCTTATCAAGTTAGCCAAGTTTATTCCAACGTGGAAAATAGTTCCAGGAAATGATATT
ATTGACATTGCAATTAAAGAACCTCACATCAGAAATCAGGTGAGTGATACTGATCTAAGA
CGCCAAATTTTACTTGTAATATCAGCTTGTTATTAACCATATATACATATGATTTATGTG
AATTTTACAGGT

Last Updated on 06.12.2018 16:11