Sequences from confirmation amplicon


Confirmation sequences for line 532B11 and hit At5g09870 are shown.
Line ID 532B11
Locus At5g09870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:3077177
Primers used for PCR MZ92 - 8474, show primer details
   
Sequence Name 21-K027364-022-532-B11C-8409
GenBank Accession LN495520 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K027364-022-532-B11C-8409
AGCCATTTACAATTGAATATATCCTGGATAAACCACAGAGTTGATGTAAGACAATCTCTC
AAGCCATTTCAAACCACCTCCATAACCATACCAAATAGGACAATGTCTGCTCAAGAAAAT
CTCAACAGACCCGAGCGCCCACCGAAGAACTTGATGAAGACGGTCAGAAAGATTGATAGG
TGCTGATCCTTTAAAGGCCGGTATCTTAGGTGTACAGTAAACCGATCTCCAGCCATGAGA
ATGCATCTTGAAACCCGTAAGGATATCCTCGGTGACAGAACCGTAGATCCACCCAATCTT
GAATAAATTTCACAAACACCATGAAAGAAATCAAAAACCATATCGCAAAATGAAAGATAA
AGTGCTAAAAAGTGTTTTAAAGAGCTCTACATTACCTCTTTTCCCCATTCGGTTTTATCT
TCGTATCCACAACTAATGACTTGGATGGCTTCTCTAAGCAGAGAAGCTGGACTCGCATTC
CTAGCAAGCCCACCATTCTCCATACCAGCAGACGCAACAAAAACAGGAGACTGTCCAAA
   
Sequence Name 22-K027364-022-532-B11C-MZ92
GenBank Accession LN495966 [GenBank]
Sequencing primer gene specific primer, MZ92: 5'-TTGGAGAAGAAGTTTGGACAGTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K027364-022-532-B11C-NZ92
ATGGTGGGCTTGCTAGGAATGCGAGTCCAGCTTCTCTGCTTAGAGAAGCCATCCAAGTCA
TTAGTTGTGGATACGAAGATAAAACCGAATGGGGAAAAGAGGTAATGTAGAGCTCTTTAA
AACACTTTTTAGCACTTTATCTTTCATTTTGCGATATGGTTTTTGATTTCTTTCATGGTG
TTTGTGAAATTTATTCAAGATTGGGTGGATCTACGGTTCTGTCACCGAGGATATCCTTAC
GGGTTTCAAGATGCATTCTCATGGCTGGAGATCGGTTTACTGTACACCTAAGATACCGGC
CTTTAAAGGATCAGCACCTATCAATCTTTCTGACCGTCTTCATCAAGTTCTTCGGTGGGC
GCTCGGGTCTGTTGAGATTTTCTTGAGCAGACATTGTCCTATTTGGTATGGTTATGGAGG
TGGTTTGAAATGGCTTGAGAGATTGTCTTACATCAACTCTGTGGTTTATCCAGGATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG
TC

Last Updated on 06.12.2018 16:11