Sequences from confirmation amplicon


Confirmation sequences for line 532E08 and hit At3g19600 are shown.
Line ID 532E08
Locus At3g19600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6811489
Primers used for PCR ML10 - 8474, show primer details
   
Sequence Name 20-K030755-022-532-E08C-8409
GenBank Accession LN495093 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K030755-022-532-E08C-8409
CATTTACATTGAATATATCATGTAGATTTCACATGAAGAAGCTTCACCTTGTCCTTGACT
TGGACCTTACGCTTATCCACTCCGTTAGGGTTCCATGTCTCTCCGAAGCAGAGAAGTATC
TAATCGAAGAAGCTGGTTCAACAACAAGGGAAGATCTATGGAAAATGAAAGTCAGAGGAG
ATCCCATATCCATAACCATAGAACACTTGGTAAAACTACGGCCATTTCTTTGCGAATTCT
TGAAAGAAGCCAACGAGATGTTCACAATGTATGTTTACACAAAGGGTACTCGCCCTTACG
CTGAAGCCATTTTGAAGCTGATTGATCCGAAGAAACTCTATTTTGGACATAGAGTGATAA
CAAGAAATGAGAGTCCTCATACGAAGACACTTGATATGGTTTTGGCTGATGAACGTGGAG
TGGTGATTGTGGATGATACACGTAAAGCTTGGCCTAAAATAACA
   
Sequence Name 59-K030755-022-532-E08C-ML10
GenBank Accession LN509895 [GenBank]
Sequencing primer gene specific primer, ML10: 5'-CTTGTTATTAGGCCAAGCTTTACG-3'
Sequence (A. th genome BLAST matches underlined) 
>59-K030755-022-532-E08C-ML10
TTCATCAGCCAAAACCATATCAAGTGTCTTCGTATGAGGACTCTCATTTCTTGTTATCAC
TCTATGTCCAAAATAGAGTTTCTTCGGATCAATCAGCTTCAAAATGGCTTCAGCGTAAGG
GCGAGTACCCTTTGTGTAAACATACATTGTGAACATCTCGTTGGCTTCTTTCAAGAATTC
GCAAAGAAATGGCCGTAGTTTTACCAAGTGTTCTATGGTTATGGATATGGGATCTCCTCT
GACTTTCATTTTCCATAGATCTTCCCTTGTTGTTGAACCAGCTTCTTCGATTAGATACTT
CTCTGCTTCGGAGAGACATGGAACCCTAACGGAGTGGATAAGCGTAAGGTCCAAGTCAAG
GACAAGGTGAAGCTTCTTCATGTTGAAATCTACATGATATATTCAATTGTAAATGGCTTC
ATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAA
AGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAA
ATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCA
ATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11