Sequences from confirmation amplicon


Confirmation sequences for line 533E08 and hit At5g22460 are shown.
Line ID 533E08
Locus At5g22460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:7444649
Primers used for PCR 24jn - 8474, show primer details
   
Sequence Name 42-K113214-0022-533-E08C-8409
GenBank Accession LN504527 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K113214-0022-533-E08C-8409
TTGAAGTAATTTTTCATTGCATTTAAGATTGTGGTTGCTTCAAAAGTCAATTTTGCGTTT
GCGTCTAGATGTGATATGCCTTTCTATAAATAGCAAAAAAAGTGTATATTTTCAATAAAA
CGTTTTCAGTTCTTGAAAAAACATAAACTCAAATAGCGTTTAGCGTTTACCATTAACAAA
TACTTTTTTTTTAAGAACCAAAGACACTGAATTCTCATTTAGCCAAAAAAAAAACCCAAA
GAAATTAAAACAATATCCTCAATTACATATAGCAAAATAAATGCAAAGTAAAGTATCAAA
ACTGTATATCTAAAAGGGTGAAAAGGGGCATGTGACATGTACAAAAGTTAATATTGAGAA
TAAAAGTGAACCTTAGGGATGGGAAATTCAGTGTCTTTGGAGCTGTTGAAGCCATGAACA
ACAATGATCTTGTAGTTAGCATTGTCTCTATCACCCCCAAATA
   
Sequence Name 42-K113215-0022-533-E08C-24jn
GenBank Accession LN504528 [GenBank]
Sequencing primer gene specific primer, 24jn: 5'-AATCTGGGGTTGATAGAGACAATG-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K113215-0022-533-E08C-24jn
CTTCACAGCTCCAAGACACTGAATTTCCCATCCCTAAGGTTCACTCTTATTCTCAATATT
AACTCTCGTACATGTCACATGCCCATTTTCACCATTTTAGATATACAGTTTTGATACTTT
ACTTTGCATTTATTTTGCTATATGTAATTGAGGATATTGTTTTAATTTCTTTGGGTTTTT
TTTTTGGCTAAATGAAAATTCAGTGTCTTTGGTTCTTAAAAAAAAAGTATTTGTTAAGGG
TAAACCCTAAACCCTATTTGAGTTTATGTTTTTTCAAAAACTGAAAACGTTTTATTGAAA
ATATACACTTTTTTTGCTATTTATAGAAGGGCATATCACATCTAGACCCAAACGCAAAAT
TGACTTTTGAAGCACCCCCAATCTTAAATGCAATGAAAAATTACTTTCAATTGTAAATGG
CTTCATGTCCGGGAAATCTACTTGGATCACCAATGAGTATGATGGTCAATATGGAGAAAA
AAAAAGAGTATTTCCCATTTTTTTTTCATTTCAAAAATGTAAATTTC

Last Updated on 06.12.2018 16:11