Sequences from confirmation amplicon


Confirmation sequences for line 534G05 and hit At2g42080 are shown.
Line ID 534G05
Locus At2g42080 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17555414
Primers used for PCR 18qw - 8474, show primer details
   
Sequence Name 48-K115837-0022-534-G05C-18qw
GenBank Accession LN506602 [GenBank]
Sequencing primer gene specific primer, 18qw: 5'-AATTTCCAAGTGGTTTCACTCTGT-3'
Sequence (A. th genome BLAST matches underlined) 
>48-K115837-0022-534-G05C-18qw
TAAACAAGTAATAGAGGAAAAGTAAATTGTTGTTACCGGCGGCTAATATTAAAATGCCCT
TTTCCGGGTCAGATCGACTGAGTCAAGCCCGAGTCACGTTACCCACATCGGCTTTTGGTT
TCTTCCTTTGCAAAACGGCGAAAAAGACACAAACTTTTCTCCTCCGATCTCACAGAGATG
AACGCCGCCATTAGAGCAGCGATTCTCCGACCACAAAGCTATTCTTCTCAGCTCATAAAC
ACCAATTGATCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGC
   
Sequence Name 16-K115833-0022-534-G05C-8409
GenBank Accession LN493570 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K115833-0022-534-G05C-8409
TCATTGATCAATTGGTGTTTATGAGCTGAGAAGAATAGCTTTGTGGTCGGAGAATCGCTG
CTCTAATGGCGGCGTTCATCTCTGTGAGATCGGAGGAGAAAAGTTTGTGTCTTTTTCGCC
GTTTTGCAAAGGAAGAAACCAAAAGCCGATGTGGGTAACGTGACTCGGGCTTGACTCAGT
CGATCTGACCCGGAAAAGGGCATTTTAATATTTACCGCCGGTAACAACAATTTACTTTTC
CTCTATTTACTTGTTTATTTTCTCTGCCTCACGAATATGTTGATATCACCGCAAACAGAG
TGAAACCCTTTGGAAAT

Last Updated on 06.12.2018 16:11