Sequences from confirmation amplicon


Confirmation sequences for line 535B06 and hit At4g28650 are shown.
Line ID 535B06
Locus At4g28650 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:14145015
Primers used for PCR EQ92 - 8474, show primer details
   
Sequence Name 31-K103250-0022-535-B06C-8409
GenBank Accession LN500123 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K103250-0022-535-B06C-8409
ATGAAGGTTAACTTCGCCTACGAAATCTCCGGTGGTTCCATCTTCAATATCCGCGGCAGA
TCTCCAGAGCTTCTTCACGGCGAGAACTGTGCTTGATCGTGACATTTCTGCTTTGTACAC
GATTCCGGTTGCTCCCATTCCGATCATGTTTGATTCTTTGATACATGCCAAGATATCGGA
AGCTGTAAACCCTAATCTATGAAACGCCATTAATCTCCAAGGCCATTCACCTTTGCTCGC
CGTCTCGTCGCCGCAGAATCCGTTAGAATACCATTTCTTGTACAGAGTTCTCGTAACAAT
GGTGAGAATCCCTAGAGCTAGAACCGATGCTATCCCAATCAACCATCCGGCGACAATTCG
TTTCCCGTGGAGGCTACNGTGACTCGATGTTGCTCTTTGGAATTTACTACACGGTGGTAG
AACGCCGCCGCAGAGACCGGAGTTTCCTCTTAGATCATCTGGATTTATGGTTTTGAGAAA
GCCGTTGATAGGGACTGGACCTGTGAGCTTGTTGTATGAGACGTTTAGGAGTTCCAGAGC
TGGAGATGTTCCGATGCTCTCTGGTAGTACTCCNGTNAAGGAATTGTTGGAGAG
   
Sequence Name 79-K103251-0022-535-B06C-EQ92
GenBank Accession LN513815 [GenBank]
Sequencing primer gene specific primer, EQ92: 5'-GTGCTAGATCTCTCCAACAATTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>79-K103251-0022-535-B06C-EQ92
GGACATCTCCGCTCTGGAATCCTAAACGTCTCATACAACAAGCTCACAGGTCCAGTCCCT
ATCAACGGCTTTCTCAAAACCATAAATCCAGATGATCTAAGAGGAAACTCCGGTCTCTGC
GGCGGCGTTCTACCACCGTGTAGTAAATTCCAAAGAGCAACATCGAGTCACAGTAGCCTC
CACGGGAAACGAATTGTCGCCGGATGGTTGATTGGGATAGCATCGGTTCTAGCTCTNGGG
ATTCTCACCATTGTTACGAGAACTCTGTACAAGAAATGGTATTCTAACGGATTCTGCGGC
GACGAGACGGCGAGCAAAGGTGAATGGCCTTGGAGATTAATGGCGTTTCATAGATTAGGG
TTTACAGCTTCCGATATCTTGGCATGTATCANAGAATCAAACATGATCGGAATGGGAGCA
ACCGGAATCGTGTACAAAGCAGAAATGTCACGATCAAGCACAGTTCTCGCCGTGAAGAAG
CTCTGGAGATCTGCCGCGGATATNGAAGATGGAACCACCGGAGATTTCGTAGGCAAAGTT
AACCTTCATATGGCTTCATGTCCGGGAAATCTACNTGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATNTC
CG
Primers used for PCR KR78 - 8474, show primer details
   
Sequence Name 42-K118809-0022-535-B06C-KR78
GenBank Accession LN504648 [GenBank]
Sequencing primer gene specific primer, KR78: 5'-CCACAATGTCTACTGATTCTCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K118809-0022-535-B06C-KR78
GCTCCATAGTACGACGCCGTAGCTGTATATGTCTATCTTCTCATCCACCTTCAAAGTGTA
TCCATACTCTGCAAATGATCAACCATATGTTGTTAGACTAAACCGGATTGTAATCAATTT
AGTAATCGGTATGGTATTTAAACCTTACCGGGAGCAATGTAACCGTAAGAACCGGCGACC
ATTGAAACGGTTTCTTTCTTCCTCGCCATCATCCTGGCGAGGCCAAAATCGGCGATTCTG
GCGTCGAGATTCGCGTCGAGCAAGATATTGTTGGATTTGATGTCTCTGTGAATCACCGGA
GGATGACAATCATGGTGGAGGTAAGCGAGGCCGTGAGCTACACCTAACGCGATGTTGTAA
CGCGAAACCCAATCAACGAGAAGTCTCCCGGCGGCGTTTTTGCCGTGAATCGCATCACCA
AGGTTTCCGTTGAGCATGAATTCATATACGATCATCATGTTTTTGTCGTTGTATAAGAAC
CCTAGGAGCCTCACTATGTTTCTGTGTCTCAATTGTAAATGGCTTCATGTCCGGGAAATC
TACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAA
TTTTTTTCAATTCAAAAATGTAG
   
Sequence Name 10-K118808-0022-535-B06C-8409
GenBank Accession LN490884 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K118808-0022-535-B06C-8409
CATTTCATTGAGACACAGAAACATAGTGAGGCTCCTAGGGTTCTTATACACGACAAAAAC
ATGATGATCGTATATGAATTCATGCTCAACGGAAACCTTGGTGATGCGATTCACGGCAAA
AACGCCGCCGGGAGACTTCTCGTTGATTGGGTTTCGCGTTACAACATCGCGTTAGGTGTA
GCTCACGGCCTCGCTTACCTCCACCATGATTGTCATCCTCCGGTGATTCACAGAGACATC
AAATCCAACAATATCTTGCTCGACGCGAATCTCGACGCCAGAATCGCCGATTTTGGCCTC
GCCAGGATGATGGCGAGGAAGAAAGAAACCGTTTCAATGGTCGCCGGTTCTTACGGTTAC
ATTGCTCCCGGTAAGGTTTAAATACCATACCGATTACTAAATTGATTACAATCCGGTTTA
GTCTAACAACATATGGTTGATCATTTGCAGAGTATGGATACACTTTGAAGGTGGATGAGA
AGATAGACATATACAGCTACGGCGTCGTACTATTGGAGCTGTTAACCGGAAGAAGACCAT
TGGAACCGGAGTTTGGAGAATCAGTAAACATTGT

Last Updated on 06.12.2018 16:11