Sequences from confirmation amplicon


Confirmation sequences for line 535E08 and hit At3g46500 are shown.
Line ID 535E08
Locus At3g46500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17121667
Primers used for PCR CH06 - 8474, show primer details
   
Sequence Name 35-K106590-0022-535-E08C-8409
GenBank Accession LN501843 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K106590-0022-535-E08C-8409
ATTTACATTGAATAATCCTGAAGCCATGTTGATTTTGGGATGTCTGACCCTTCAAACGGA
TAGTGTAATGGGGCTCCAGGTATTACGCGATTTTCACCATAGTCATATAACTTTTGATGT
GACCAGGTTTGGTTATAAAAATTTGTCTTATGGCAGATATGCAAGGATAGAGACGTGAAG
CCTAGGAAGTGGGAATATATACTATCGATAAAAGGGTATAATGAGATTTTTTTTTAGGTC
CATGCTCCAATTTACACTAACTCCCATAACAAAAAATTGTTTTTATATGGTTTGTTTTCC
TATACAGGGCATATATCGTGAATATTGGTGACTTGCTAGAGCGTTGGAGCAATGGCATTT
TTAAGTAAATAATTGTTGTTTGAGCTAGCTAATTTATCCTTTTGCATTGTGATTGATTTT
TGCAAGTAAAGTTTTCTTGCTAACATTTATCTCACATGTTTTTTTTTACACATAGATCCA
CATTGCATCGGGTACTTGGGAATGGTCAAGACCGATATTCCGTAAGT
   
Sequence Name 51-K106591-0022-535-E08C-CH06
GenBank Accession LN507513 [GenBank]
Sequencing primer gene specific primer, CH06: 5'-AGACTTACGGAATATCGGTCTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>51-K106591-0022-535-E08C-CH06
GATCATGTGTAAAAAAACATGTGAGATAAATGTTAGCAAGAAAACTTTACTTGTAAAAAT
CAATCACAATGCAAAAGGATAAATTAGCTAGCTCAAACAACAATTATTTACTTAAAAATG
CCATTGCTCCAACGCTCTAGCAAGTCACCAATATTCACGATATATGCCCTGTATAGGAAA
ACAAACCATATAAAAACAATTTTTTGTTATGGGAGTTAGTGTAAATTGGAGCATGGACCT
AAAAAAAAATCTCATTATACCCTTTTATCGATAGTATATATTCCCACTTCCTAGGCTTCA
CGTCTCTATCCTTGCATATCTGCCATAAGACAAATTTTTATAACCAAACCTGGTCACATC
AAAAGTTATATGACTATGGTGAAAATCGCGTAATACCTGGAGCCCCATTACACTATCCGT
TTGAGGGATCAGACATCCCAAAATCAACATGGCTTCAGGATATATTCAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT

Last Updated on 06.12.2018 16:11