Sequences from confirmation amplicon


Confirmation sequences for line 535E12 and hit At1g02750 are shown.
Line ID 535E12
Locus At1g02750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:603793
Primers used for PCR 25wj - 8474, show primer details
   
Sequence Name 83-K118544-0022-535-E12C-25wj
GenBank Accession LN514356 [GenBank]
Sequencing primer gene specific primer, 25wj: 5'-TGACAACAGTCCCTGTACAAACTC-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K118544-0022-535-E12C-25wj
CCGATAAAGGACACGACGATGTACCCTCTTTTGCTGCTGACGAATCCTTAATCGGATTCT
TGTCTTCTACACTTGAATCAGGCAGCACAAGCTTGGTCTGATTTGGTAATGCAGAATTGT
TGATGAACGATAAAAATTGATCGGAAACATGTTTAGAGGGATGCTGATGATGATTCATAG
ATGGTGGCAACTCATCAAGATGAGGTTGAAGATATTTATCAGATAAATATGGATCTTCCC
TGTAAGAAAAAGAAAAAAATTGTAATTACCAATATACAATTGTAAATGTAAATGTAAATG
TAAATGTAATTGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCA
   
Sequence Name 63-K118543-0022-535-E12C-8409
GenBank Accession LN511086 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>63-K118543-0022-535-E12C-8409
CCTTTACATCGAATATATTCAATTACATTTACATTTACATTTACATTTACAATTGTATAT
TGGTAATTACAATTTTTTTCTTTTTCTTACAGGGAAGATCCATATTTATCTGATAAATAT
CTTCAACCTCATCTTGATGAGTTGCCACCATCTATGAATCATCATCAGCATCCCTCTAAA
CATGTTTCCGATCAATTTTTATCGTTCATCAACAATTCTGCATTACCAAATCAGACCAAG
CTTGTGCTGCCTGATTCAAGTGTAGAAGACAAGAATCCGATTAAGGATTCGTCAGCAGCA
AAAGAGGGTACATCGTCGTGTCCTTTATCGGATAGTGATAAATTGGAGAAGGCAAAGAAG
TGTGAGTTTGTACAGGATCGTGTTGTC

Last Updated on 06.12.2018 16:11