Sequences from confirmation amplicon


Confirmation sequences for line 535H06 and hit At3g06760 are shown.
Line ID 535H06
Locus At3g06760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2133674
Primers used for PCR IN20 - 8474, show primer details
   
Sequence Name 57-K033552-022-535-H06C-8409
GenBank Accession LN509332 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K033552-022-535-H06C-8409
CATTTACAATTGAATATCCGAAGAAAAAGAACACATTTTTAAGTCCACGTTTAAGAGGAA
ACAGAGATGTAGCTAAAAAAAGTAGCAGTCTGGATCATGAAAAGATCACTAATGTTACCG
ACCGAATAAACGAAATATTCCAAGATTGGATTCACAAGGCTGCTAAATGGATTACAGTGC
TCATCTGATAAGAAGAAACAACAGAGGTAGCCAGAGAACAGTTCATCATCACGAAGAACA
GAAAAAAAAAATATTCCAACATGTTCAATTTTTCTAACTATAAACCAAAAATTGCAGCAT
TGGATCACAACTAAGGCAAAGAACTGATCAACCTAAGAATCAAACAAAGCATCAAGATAT
CTATCCTAAAGCTAAAGCAGGGTTTGATAGATGATCAATTATAATTACCCCATTCTTGGC
TTCAACAGGATGTCTTTCATCA
   
Sequence Name 57-K033552-022-535-H06C-IN20
GenBank Accession LN509333 [GenBank]
Sequencing primer gene specific primer, IN20: 5'-TTGATGAAGAACATCCTGTTGAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K033552-022-535-H06C-IN20
CAACCCTGCTTTAGCTTTAGGATAGATATCTTGATGCTTTGTTTGATTCTTAGGTTGATC
AGTTCTTTGCCTTAGTTGTGATCCAATGCTGCAATTTCTGGTTTATAGTTAGAAAAATTG
AACATGTTGGAATATTTTTTTTTTCTGTTCTTCGTGATGATGAACTGTTCTCTGGCTACC
TCTGTTGTTTCTTCTTATCAAATGAGCACTGTAATCCATTTAGCAGCCTTGTGAATCCAA
TCTTGGAATATTTCGTTTATTCGGTCGGTAACATTAGTGATCTTTTCATGATCCAAACTG
CTACTTTTTTTAGCTACATCTCTGTTTCCTCTTAAACGTGGACTTAAAAATGTGTTCTTT
TTTCTTCGGATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCACCAA
TGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCA
AAAATGTAAATGTCCGCACCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAA
TTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
TTATTTCGACGTGTCTACATTC

Last Updated on 06.12.2018 16:11