Sequences from confirmation amplicon


Confirmation sequences for line 536D02 and hit At3g16150 are shown.
Line ID 536D02
Locus At3g16150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:5472007
Primers used for PCR 19kk - 8474, show primer details
   
Sequence Name 42-K113817-0022-536-D02C-19kk
GenBank Accession LN504535 [GenBank]
Sequencing primer gene specific primer, 19kk: 5'-GAATAAATGGACGGTGGAGATTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>42-K113817-0022-536-D02C-19kk
CTTCTTGGCCACTTGAGCATTCCTACGTTGTCGTCCGTGACAAAGTACTCGTTGTCCACA
ATTTCAACTCCCTTCTCATTAAACCGGCTTCGGTCAGAAGATTTATCTAACCAAGTGGTT
TAGAATAAAAAGTGGAAGGGTAAAATAGTAAATAAACACCTGTTTGCGGGCGAAATCCTC
TGCACCTGAGAAAGCAAGGTAAGAGTGGGGAGATTTGTCCATGACGAGACGAGCAAGAGA
TATAGGATTTTTCACGGTGGTTATCCCCGAAACGGCACCGCATCGTCTCTTCGTACCGTC
CATAATGCTAGCTTCCATCTCAACCGTTCCTTTTTCCGTCAAAGCAGATCCACGGCCTGA
ATTAAACAGAGGATCCGTCTCCAATTCTCTAATCTGTGTTAATCAATCGTTTAGTATGAG
TATGAGTATACATGGCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCA
GCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAA
TTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 16-K113816-0022-536-D02C-8409
GenBank Accession LN493509 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>16-K113816-0022-536-D02C-8409
GAGCCATGTATACTCATACTCATACTAAACGATTGATTACCATAGATTAGAGAATTGGAG
ACGGATCCTCTGTTTAATTCAGGCCGTGGATCTGCTTTGACGGAAAAAGGAACGGTTGAG
ATGGAAGCTAGCATTATGGACGGTACGAAGAGACGATGCGGTGCCGTTTCGGGGATAACC
ACCGTGAAAAATCCTATATCTCTTGCTCGTCTCGTCATGGACAAATCTCCCCACTCTTAC
CTTGCTTTCTCAGGTGCAGAGGATTTCGCCCGCAAACAGGTGTTTATTTACTATTTTACC
CTTCCACTTTTTATTCTAAACCACTTGGTTAGATAAATCTTCTGACCGAAGCCGGTTTAA
TGAGAAGGGAGTTGAAATTGTGGACAACGAGTACTTTGTCACGGACGACAACGTAGGAAT
GCTCAAGTTGGCCAAGGAAGCTAACTCCATCTTGGTACGTTTTTGACTTAAATCTCCACC
GTCNATTTATT

Last Updated on 06.12.2018 16:11