Sequences from confirmation amplicon


Confirmation sequences for line 536H01 and hit At2g20470 are shown.
Line ID 536H01
Locus At2g20470 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:8827389
Primers used for PCR BK03 - 8474, show primer details
   
Sequence Name 77-K104542-0022-536-H01C-BK03
GenBank Accession LN513548 [GenBank]
Sequencing primer gene specific primer, BK03: 5'-AGCATTATTTGGTGACACTGTCTG-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K104542-0022-536-H01C-BK03
TTTTCTCAATGAAATGCTATGTAGGTGGTCTCTTGGTGCTATAATGTACGAAATGCTAGT
AGGATATCCGCCGTTTTATTCAGATGATCCTATGTCAACCTGTAGAAAGGTAATTTTCGC
AAGAATGAATTGAAATTTGTTAACCTTCCAGTGACACTGACATGACATCAAACTCTTGTT
CCTGCTTTTGGTTGCAGATAGTAAACTGGAAAAGTCATTTGAAGTTTCCAGAGGAAGCAA
TATTGTCAAGGGAAGCAAAGGATCAGCAAAGGGTATGATGGCGCTCCCCGGGTAACATAT
ATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATG
GTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGAT
GTCCGCAGCGTTATTA
   
Sequence Name 93-K104543-0022-536-H01C-8409
GenBank Accession LN515254 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>93-K104543-0022-536-H01C-8409
TTACAATTGAATATTGTTACCCGGGGAGCGCCATCATACCCTTTGCTGATCCTTTGCTTC
CCTTGACAATATTGCTTCCTCTGGAAACTTCAAATGACTTTTCCAGTTTACTATCTGCAA
CCAAAAGCAGGAACAAGAGTTTGATGTCATGTCAGTGTCACTGGAAGGTTAACAAATTTC
AATTCATTCTTGCGAAAATTACCTTTCTACAGGTTGACATAGGATCATCTGAATAAAACG
GCGGATATCCTACTAGCATTTCGTACATTATAGCACCAAGAGACCACCTACATAGCATTT
CATTGAGAAAACTGCATTAGCGTCCTATTCAAAGCACAAATGCAGACAGTGTCACCAAAT
AATGCTATGTAGGTGGTCTCTTGGTGCTATAATGTACGAAATGCTAGTAGGATATCCGCC
GTTTTATTCAGATGATCCTATGTCAACCTGTAGAAAGGTAATTTTCGCAAGAATGAATTG
AAATTTGTTAACCTTCCAGTGACACTGACATGACATCAAACTCTTGTTCCTGCTTTTGGT
TGCAGATAGTAAACTGGAA

Last Updated on 06.12.2018 16:11