Sequences from confirmation amplicon


Confirmation sequences for line 539H01 and hit At5g65683 are shown.
Line ID 539H01
Locus At5g65683 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:26261614
Primers used for PCR 12mp - 8474, show primer details
   
Sequence Name 53-K114277-0022-539-H01C-12mp
GenBank Accession LN508227 [GenBank]
Sequencing primer gene specific primer, 12mp: 5'-TATTACTCTGCATCCACACACACA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K114277-0022-539-H01C-12mp
TAAGAACTTGATTGAATTCAAAAGTAACTACCTCGGTTGAGAAGAAGCGTGGCACGGAGG
AGAGAGACGCCGCCGCCGCCTTGAGAGGAAGAAGAAGATGATGATTTAGGAGAAGAAGGG
TTAGAGAAAAACTTGGGACTTGAGCTGTTACTAGTCCTTGGAGTAGCATTGCTCGTAGTC
CTGCAATGGAGTTTGGGACTAGTTGGAAGAGATGGGATAGTGACTGATGTGGAAGCTGAG
GAGCGGCATCCAGTGCCGCTTCCGCCGCGTGATCGGAGACGAGGAGTTGAAGTCAGGATA
TATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCACGGTTTATT
   
Sequence Name 23-K114276-0022-539-H01C-8409
GenBank Accession LN496651 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K114276-0022-539-H01C-8409
TCCTGACTTCAACTCCTCGTCTCCGATCACGCGGCGGAAGCGGCACTGGATGCCGCTCCT
CAGCTTCCACATCAGTCACTATCCCATCTCTTCCAACTAGTCCCAAACTCCATTGCAGGA
CTACGAGCAATGCTACTCCAAGGACTAGTAACAGCTCAAGTCCCAAGTTTTTCTCTAACC
CTTCTTCTCCTAAATCATCATCTTCTTCTTCCTCTCAAGGCGGCGGCGGCGTCTCTCTCC
TCCGTGCCACGCTTCTTCTCAACAAGGTAGTTACTTTTGATTCAATCAAGTTCTTAATTT
GAAAGACATAATCTAAAGCTCCGTTTTGTGTGTGTGTGGATCNAAAGTAAT

Last Updated on 06.12.2018 16:11