Sequences from confirmation amplicon


Confirmation sequences for line 540D06 and hit At1g76900 are shown.
Line ID 540D06
Locus At1g76900 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:28884073
Primers used for PCR 03ar - 8474, show primer details
   
Sequence Name 05-K106771-0022-540-D06C-8409
GenBank Accession LN488372 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K106771-0022-540-D06C-8409
TCGGACTGAGCCTTTCTCGGCGGCTTGTTCTTAAGCACAAGTGGTGGCGAATTCCGCTCT
TTCCCTTCTTCTATACCACTTCTTGGTCTTCGCTTAACGGGCCAAGAATGTCGGAAAACC
GTGCACTGCTAAAATCATTCGAGTAATCGTAAGTGATTTTTCTCGATGACGAAGAGGTAA
TGCTGCGGAACGATTCGTCGAGAATAGAGCGCGGGACAATGATATCGGGCTGTCCAGGCA
CAGTTCCGCCTTCCGCAAGGGAAGAGGCGGGAATTGAGTTCATCGCACAATGCATTCTCC
TCGGACCACGGGTACCAAGAACGTTTAGCTCATAAGAAACCTGCGCAATTTTGTAGCTCC
CACTAGGTACTTTGGGAGAGACTCTCTTTGAGTAGAATCTGCGGCTAAGACCTACCGGTT
GGACCGCTCGAGCGATGTTGCTGTTGTATGCTGGTTGTGTATCGTATATTATAAACTTCG
TCCCCAGAAAATTAGACCTGCCAAAGAGGAGATAATCACGCGTTAAGCCGGAAACATTAG
CACATTCGCTTTCTAAATGTCTTATAACTAGGCAAAGTCTTTCCTATTACCTGATTTTGC
CAATGTAGGTATTGCTTGATCTCGAAATGGTGTCGGCGTGCATAGAGATCACGTACTCGG
TGTATGTAGTTCTTCTTATGCGTTTTGCAGAAAGAAGAAACTTTCCATTCTCAACCAACA
AAGCTGCAAATATTCATGCTTTCAGATTTGCTTTGGAGAACAGAGAAAAACTTGGAACAA
AAAC
   
Sequence Name 21-K106772-0022-540-D06C-03ar
GenBank Accession LN495680 [GenBank]
Sequencing primer gene specific primer, 03ar: 5'-TCATTTTCTCTGTTTTTGTTCCAA-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K106772-0022-540-D06C-03ar
TGAAGCTGAATATTTGCAGCTTTGTTGGTTGAGAATGGAAAGTTTCTTCTTTCTGCAAAA
CGCATAAGAAGAACTACATACACCGAGTACGTGATCTCTATGCACGCCGACACCATTTCG
AGATCAAGCAATACCTACATTGGCAAAATCAGGTAATAGGAAAGACTTTGCCTAGTTATA
AGACATTTAGAAAGCGAATGTGCTAATGTTTCCGGCTTAACGCGTGATTATCTCCTCTTT
GGCAGGTCTAATTTTCTGGGGACGAAGTTTATAATATACGATACACAACCAGCATACAAC
AGCAACATCGCTCGAGCGGTCCAACCGGTAGGTCTTAGCCGCAGATTCTACTCAAAGAGA
GTCTCTCCCAAAGTACCTAGTGGGAGCTACAAAATTGCGCAGGTTTCTTATGAGCTAAAC
GTTCTTGGTACCCGTGGTCCGAGGAGAATGCATTGTGCGATGAACTCAATTCCCGCCTCT
TCCCTTGCGGAAGGCGGAACTGTGCCTGGACAGCCCGATATCATTGTCCCGCGCTCTATT
CTCGACGAATCGTTCCGCAGCATTACCTCTTCGTCATCGAGAAAAATCACTTACGATTAC
TCGAATGATTTTAGCAGTGCACGGTTTTCCGACATTCTTGGCCCGTTAAGCGAAGACCAA
GAAGTGGTATTAGAAGAAGGGAAAGAGCGGAATTCGCCACCACTTGTGCTTAAGAACAAG
CCGCCGAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGG
TCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
TCC

Last Updated on 06.12.2018 16:11