Sequences from confirmation amplicon


Confirmation sequences for line 540H02 and hit At5g50120 are shown.
Line ID 540H02
Locus At5g50120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20383306
Primers used for PCR 77rq - 8474, show primer details
   
Sequence Name 57-K116204-0022-540-H02C-77rq
GenBank Accession LN509514 [GenBank]
Sequencing primer gene specific primer, 77rq: 5'-ACTCTTGTATGTCACACAGCATCC-3'
Sequence (A. th genome BLAST matches underlined) 
>57-K116204-0022-540-H02C-77rq
TTACCAGGCTCCACGACGGGGTTGTTAGATTATGGAATGCAAACACGTTGGAAACATTAG
CCGAAGCATCAAGCAACGGCGATGTAATAACAGGGGAGAGAGGAGGTGGAGGAGCAGTGA
AATCTTTGGTAATCTTGGCTGATAAACTCTTTACAGCACACCAAGATCATAAGATACGTG
TGTGGAAGATAAACGACGTCGTTGAAGAAGACGTGGGTGGTAAAAAGTACATGCATTTAG
CGACGATGCCAACGATTAGCGATCGTTTTGCAAAGTGTTTGATGCCAAAGAACCAAGTTG
AAATAAGGAGGCATAAGAAAGCTTCATGGGTTCACCAATTGTAAATGGCTTCATGTCCGG
GAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAAT
TACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGATTATTATA
   
Sequence Name 21-K116203-0022-540-H02C-8409
GenBank Accession LN495808 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K116203-0022-540-H02C-8409
ATTTCATTGGTGACCCATGAAGCTTTCTTATGCCTCCTTATTTCAACTTGGTTCTTTGGC
ATCAAACACTTTGCAAAACGATCGCTAATCGTTGGCATCGTCGCTAAATGCATGTACTTT
TTACCACCCACGTCTTCTTCAACGACGTCGTTTATCTTCCACACACGTATCTTATGATCT
TGGTGTGCTGTAAAGAGTTTATCAGCCAAGATTACCAAAGATTTCACTGCTCCTCCACCT
CCTCTCTCCCCTGTTATTACATCGCCGTTGCTTGATGCTTCGGCTAATGTTTCCAACGTG
TTTGCATTCCATAATCTAACAACCCCGTCGTTGGAGCCTGTGTAAAGTCTCTTTCCTGCA
AGAGTTAGAGATGAAATGTAGGATGCTGTGTGAATTACAAGAGTA

Last Updated on 06.12.2018 16:11