Sequences from confirmation amplicon


Confirmation sequences for line 541B12 and hit At5g15620 are shown.
Line ID 541B12
Locus At5g15620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5082716
Primers used for PCR CH09 - 8474, show primer details
   
Sequence Name 44-K106590-0022-541-B12C-8409
GenBank Accession LN505195 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K106590-0022-541-B12C-8409
TTGAATTATCCTGAGCAAATCCATAGACACCAATTTCAGACCCAATCTCTTTATACGGTA
ACTGGCAGGTTCAGCTAATAATCCATCAACAAAATTCTTGAAACTTTCCCAATGATGGAA
AACAACGTTTCGTTTGATAGTAACCAAATTCTGAAAGTTCTTGGAGACAAACTTTAAGCA
CGTACCCTCCTTTGCCGAAAGAAAAGAAACGATGTGGTAAATAACATCGTCTGGTAAATT
GCTGAATCTATCCATACTTATCCCAAACCCTAGAGAACCGATCGATCAATACCAAAAAAA
CTTCAATTTTCTAATCGAAACAAGAAATCTGAAGCCAACACGAGATTCAATATTGAAATT
GTGATACGATCGACGACGCCAATTCGAGAGCTTTCTGACGCGAATCAAAGAGGAATTGAC
GAATAACTAGAGCGGAGTTGTGACATTTATTGAAAGCAAAGAAAAGAAACGAACACGACT
GGGGTAATTTGAATCCACT
   
Sequence Name 60-K106591-0022-541-B12C-CH09
GenBank Accession LN510270 [GenBank]
Sequencing primer gene specific primer, CH09: 5'-TAGTGGATTCAAATTACCCCAGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K106591-0022-541-B12C-CH09
TGTCACAACTCCGCTCTAGTTATTCGTCAATTCCTCTTTGATTCGCGCCATAAAGCTCTC
GAATTGGCGTCGTCGATCGTATCACAATTTCAATATTGAATCTCGTGTTGGCTTCAGATT
TCTTGTTTCGATTAGAAAATTGAAGTTTTTTTGGTATTGATCGATCGGTTCTCTAGGGTT
TGGGATAACTATGGATAGATTCTGCAATTTACCAGACAATGTTATTTACCACAATCCTTT
CTTTTCTTTCGGCAAAGGATGCTACATGCTTAAAGTTTGTCTCCAAGAACTTTCAGAATT
TGGTCACTACCAAAAGAAAC

Last Updated on 06.12.2018 16:11