Sequences from confirmation amplicon


Confirmation sequences for line 541G11 and hit At3g61630 are shown.
Line ID 541G11
Locus At3g61630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22805740
Primers used for PCR SW04 - 8474, show primer details
   
Sequence Name 55-K027489-022-541-G11C-8409
GenBank Accession LN508693 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>55-K027489-022-541-G11C-8409
TAGACTATCGTACGCTATAGCAGCTTCCTCCGCCGTGACAAAAGTCCCAAGCCAAATCCG
AGTACGGCTCGACGGATCTCTAATCTCCGCCGCGAATTTTCCCCAAGGTCTCTGTCTCAC
GCCTCTGTACTTTTTAGGCTTCACCGACGTCGACACGTCATCTTCAACCACTACGCTTTC
CTCCTCCTTGTGCTTACCCTTTCCTCGCCGCGGTGGAGGAAGAAGATTCACCTTGGCAGA
ATCTAATCTCCTTCACGTATCGTTTCACTCTTGGAACCACCGTGACGTTGTTGTTGTCGT
CGTCGTCGCTAGACGAGTCAGTAGCGAAAGGATCAGTAACAGTGATACGGACCAGTCTCG
GAGACCCGTTAGATGGTGTAGCTGCTACGTTTGTGACCGTACGATTCTCTGTGAACTTCA
CTCGTCTCGTTCGTCTCTCCATAGAGAGAGAGAGAGAGAGGGAGTTAAAAAATGAAGAGA
GGTTTTTTTCACT
   
Sequence Name 56-K027489-022-541-G11C-SW04
GenBank Accession LN508990 [GenBank]
Sequencing primer gene specific primer, SW04: 5'-CTGCAAATTCAAAACAGTGAAAAA-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K027489-022-541-G11C-SW04
TTTTACTCCCTCTCTCTCTCTCTCTCTATGGAGAGACGAACGAGACGAGTGAAGTTCACA
GAGAATCGTACGGTCACAAACGTAGCAGCTACACCATCTAACGGGTCTCCGAGACTGGTC
CGTATCACTGTTACTGATCCTTTCGCTACTGACTCGTCTAGCGACGACGACGACAACAAC
AACGTCACGGTGGTTCCAAGAGTGAAACGATACGTGAAGGAGATTAGATTCTGCCAAGGT
GAATCTTCTTCCTCCACCGCGGCGAGGAAAGGTAAGCACAAGGAGGAGGAAAGCGTAGTG
GTTGAAGATGACGTGTCGACGTCGGTGAAGCCTAAAAAGTACAGAGGCGTGAGACAGAGA
CCTTGGGGAAAATTCGCGGCGGAGATTAGAGATCCGTCGAGCCGTACTCGGATTTGGCTT
GGGACTTTTGTCACGGCGGAGGAAGCTGCTATAGCGTACGATAGTCTACATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATG
AAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATA
AACAAATTATTCTAATTCGGAAATCTTTATNTCGACGTGTCTAC
Primers used for PCR 01jb - 8474, show primer details
   
Sequence Name 18-K118578-0022-541-G11C-01jb
GenBank Accession LN494520 [GenBank]
Sequencing primer gene specific primer, 01jb: 5'-TTCAGTTTCGTTTTCTCCTTGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K118578-0022-541-G11C-01jb
TCGAGAAACAAAAGGGTCAGGGGCAATTTTCGGTATTCCAAAAACATTCGCCGGCGAGAG
ATAAATCCGGTAACAGATACGGATCCGGGAACACCAAACCCGCCGACGATTCTTCTTCTT
CTTTTATAACCGTCGACTTTCTCTCCGGAGATAGCTCGATCGCTTCAATCTCATGCTCTG
TTTCCTCGTTGACGTTGAATCTTAGAACAGAGGTCGGTGAATGAAGGCTCTGCCGAGAAT
CTCTACCGTAATCGCAGGCGGAAACCGTTTGGAGATCGATAACCGGCGTTGGCGTCGGCG
GAGTTAGGAAATTCGTGAGCGCTTTAGGTCCTTTGAGATGACTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGT
   
Sequence Name 02-K118577-0022-541-G11C-8409
GenBank Accession LN487244 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K118577-0022-541-G11C-8409
CGCTCACGAATTTTCCAACTCCGCCGACGCCAACGCCGGTTATCGATCTCCAAACGGTTT
CCGCCTGCGATTACGGTAGAGATTCTCGGCAGAGCCTTCATTCACCGACCTCTGTTCTAA
GATTCAACGTCAACGAGGAAACAGAGCATGAGATTGAAGCGATCGAGCTATCTCCGGAGA
GAAAGTCGACGGTTATAAAAGAAGAAGAAGAATCGTCGGCGGGTTTGGTGTTCCCGGATC
CGTATCTGTTACCGGATTTATCTCTCGCCGGCGAATGTTTTTGGGATACCGAAATTGCCC
CTGACCTTTTGTTTCTCGATGAAGAAACCAAAATCCAATCAACGTTGTTACCAAACACAG
AGGTTTCGAAACAAGGAGAAAGAAAAAACTGA

Last Updated on 06.12.2018 16:11