Genotyping and primer details


Genotyping and primer details for 541G11 - At3g61630
Line ID 541G11
Locus At3g61630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22805740 of line 541G11 Chr3:22805740
   
Insertion allele amplicon 8474 - 01jb
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 01jb: 5'-TTCAGTTTCGTTTTCTCCTTGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22806131
   
Insertion allele amplicon 8474 - SW04
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer SW04: 5'-CTGCAAATTCAAAACAGTGAAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22805208
   
wt amplicon (over InsPos) 01jb - SW04
wt amplicon start Chr3:22805208
wt amplicon end Chr3:22806131
wt amplicon size 924 bp
Locus specific primer SW04: 5'-CTGCAAATTCAAAACAGTGAAAAA-3'
2nd locus specific primer 01jb: 5'-TTCAGTTTCGTTTTCTCCTTGTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11