Sequences from confirmation amplicon


Confirmation sequences for line 544A07 and hit At1g12760 are shown.
Line ID 544A07
Locus At1g12760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4350426
Primers used for PCR HP73 - 8474, show primer details
   
Sequence Name 72-K033553-022-544-A07C-8409
GenBank Accession LN512662 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K033553-022-544-A07C-8409
TTACAATTGAATATATCCTACGCATGAACAGTGGAAATGGTGTCCACAAGGTAGTTCCCT
TAGCTCTGTTCCGTCTTCATATGCAGAGAGACAGATGCAACATTCCTGTAATGCAGAATC
ATAAGAACCAAGAAGCATAATTATCTATCTAGGGGTTATTCGTCAAGGAGGAGAGATTGG
TGTGTTATAGTGTATACTTACTGCATCTTCTTGCAAAAGAGTATGTTCAATGGGTGAATC
TGTACCACACTCAGTCATTATTCCCTCGGTAGTTCCTTGGGCTTCATCATTAGTGTGTTT
GTTGGCATCACCTAGTTTGCGAAACTTGAATTTGGTGAGCTGCTCAATGTCTTCTTTTGA
AGCCCCTTCCTGCATTTTTATCATGTTGTGTTAAAACAAATAGCACAATGTGGCGACTTA
TGATATAAGATGGAATTGGTTATGAGAAGTACCTGATCAGCAACAGCGTACAAAACTGCA
ATGATGCATGGCAGGCAACAGCAAACCGCAATTCCAATAACGCAGGCCAACGCAACACAG
AAGACAACAAAGACANCATCG
   
Sequence Name 72-K033553-022-544-A07C-HP73
GenBank Accession LN512663 [GenBank]
Sequencing primer gene specific primer, HP73: 5'-TTCGATGTGTTCTTTGTTGTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>72-K033553-022-544-A07C-HP73
GATTGCGGTTTGCTGTTGCCTGCCATGCATCATTGCAGTTTTGTACGCTGTTGCTGATCA
GGTACTTCTCATAACCAATTCCATCTTATATCATAAGTCGCCACATTGTGCTATTTGTTT
TAACACAACATGATAAAAATGCAGGAAGGGGCTTCAAAAGAAGACATTGAGCAGCTCACC
AAATTCAAGTTTCGCAAACTAGGTGATGCCAACAAACACACTAATGATGAAGCCCAAGGA
ACTACCGAGGGAATAATGACTGAGTGTGGTACAGATTCACCCATTGAACATACTCTTTTG
CAAGAAGATGCAGTAAGTATACACTATAACACACCAATCTCTCCTCCTTGACGAATAACC
CCTAGATAGATAATTATGCTTCTTGGTTCTTATGATTCTGCATTACAGGAATGTTGCATC
TGTCTCTCTGCATATGAAGACGGAACAGAGCTAAGGGAACTACCTTGTGGACACCATTTC
CACTGTTCATGCGTAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTTCGACGTGTCTACAT

Last Updated on 06.12.2018 16:11