Sequences from confirmation amplicon


Confirmation sequences for line 544B06 and hit At5g49910 are shown.
Line ID 544B06
Locus At5g49910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20305497
Primers used for PCR IF56 - 8474, show primer details
   
Sequence Name 71-K101386-0022-544-B06C-8409
GenBank Accession LN512525 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K101386-0022-544-B06C-8409
GCCTTTACATTGAATATATCCTGATAAACACCACACTTCCAACTTCTAAGTCAGAAGTCT
TCTCAACTGCTGCAGATGGACAGACAAGCGTTGAGATTAATGTGTTACAGGGTGAGAGAG
AGTTCGTAAGAGATAACAAGTCTATTGGTAGCTTCCGTCTTGATGGTATTCCACCTGCAC
CACGTGGAGTTCCACAAATCGAGGTCAAATTCGACATTGATGCCAATGGAATTCTATCTG
TCAGTGCTTCGGACAAAGGTACAGGAAAGAAGCAGGACATCACCATTACTGGTGCTAGTA
CCTTACCAAAGGATGAGGTGTGTACAGTTATCAAACTTGGTTCACTTATGTCAGATATGT
AATTTTTTTTCTTCTTCATATCANTGATTCATATTGGTTGTATGATGTTGCAGGTAGACA
C
   
Sequence Name 71-K101392-0022-544-B06C-IF56
GenBank Accession LN512526 [GenBank]
Sequencing primer gene specific primer, IF56: 5'-TGTGTCTACCTGCAACATCATACA-3'
Sequence (A. th genome BLAST matches underlined) 
>71-K101392-0022-544-B06C-IF56
GAGAGAAAAAATTCATATCTGACATAAGTGAACCAAGTTTGATAACTGTACACACCTCAT
CCTTTGGTAAGGTACTAGCACCAGTAATGGTGATGTCCTGCTTCTTTCCTGTACCTTTGT
CCGAAGCACTGACAGATAGAATTCCATTGGCATCAATGTCGAATTTGACCTCGATTTGTG
GAACTCCACGTGGTGCAGGTGGAATACCATCAAGACGGAAGCTACCAATAGACTTGTTAT
CTCTTACGAACTCTCTCTCACCCTGTAACACATTAATCTCAACGCTTGTCTGTCCATCTG
CAGCAGTTGAGAAGACTTCTGACTTAGAAGTTGGAAGTGTGGTGTTTATCAGGATATATT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAANAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATGTAGATGTC
CGCAGCG

Last Updated on 06.12.2018 16:11