SimpleSearch - Line and FST details


Line specific information

 
Line ID 544B06
Vector Used pAC161
Line Availability available as T3 set from NASC (N452146)
Segregation Analysis 50:49:36
Confirmed for Hit At5g49910
Parent of DUPLO pair none
Parent of pair(s) 3404, 3542, 3577
Note

Gene hit At5g49060

 
Sequence (A. th genome BLAST matches underlined) 
>42-K022309-022-544-B06-8409
TCCGGAATGAGCCATTTACATATGAATAATCCTGATCAGCTTCTGACTGATCAGCTTCTC
CTTCTCTCTTCCATGGCGATTCTCGTCGTCTGAATTAGGTACTTTTCTAAATCTTACACC
TTCTATTCAGTACGCGAAATTGACTCTTCTTTGCTGTCTTGACCTATGGGATCCTCCCTA
TAGTGAGATTTGGGATCCTCCCTATCATACCGCTGCGGACATCAACGTTTTCGATAAAAG
GTAATTACATATGGCATCCTCACTATATCGAGATATTGAAACCTCCATAATAACCTGCGG
CGCAAACCAAAATTTCCCACATAGACACACTTCTTATAGCCTCCGACACGAAAAGATAAT
GGAGTAAATAATTTATAAATTATGGGCGCCATTTAGTTAAACTGAAGAGCAGAGAATATT
TTAAAACAATCGAT
GenBank Accession BX650200 [GenBank]
Graphic View Graphic view of gene At5g49060
Predicted Position of Insertion Chr5:19886493 - go to primer design
BLAST e Value 3e-62
Hit Clone Code (BAC ID) K19E20
Hit Gene Code At5g49060 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation DnaJ heat shock amino-terminal domain protein (DUF1977)
Insertion Classification TS2TE (5')
Confirmation Status failed

Gene hit At5g49910

 
Sequence (A. th genome BLAST matches underlined) 
>42-K022299-022-544-B06-8409
TGAGCCATTTACATTAGTCATTTACGGATCTACCTTCGCTACCGATGCGTGCTATGGGAT
CCTCCCTATAGTGAGACATGGACAGACCATCGTTGAGATTAATGTGTTACACGGTGAGAG
AGAGTTCGTATTAGATAACAAGTCTATTGGTAGCTTCCGTCTTGATGGTATTCCACCTGC
ACCTCGTGGAGTTCCACAAATCGAGGTCAAATTCGACATTGATGCCAATGGAATTCTATC
TGTCAGTGCTTCGGACAAGGGTACAGGAAATAAGCAGGACATCTCCATTACTGGTGCTAT
GGGAACC
GenBank Accession BX650199 [GenBank]
Graphic View Graphic view of gene At5g49910
Predicted Position of Insertion Chr5:20305512 - go to primer design
BLAST e Value 3e-101
Hit Clone Code (BAC ID) K9P8
Hit Gene Code At5g49910 [Araport] [TAIR] [MIPS] [SIGnAL]
Gene Annotation chloroplast heat shock protein 70-2
Insertion Classification CDSi
Confirmation Status confirmed, show confirmation sequences
Primer and wt-amplicons show primer details


Last Updated on 10.06.2021 13:37