Sequences from confirmation amplicon


Confirmation sequences for line 544E01 and hit At3g55110 are shown.
Line ID 544E01
Locus At3g55110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20425021
Primers used for PCR 57wj - 8474, show primer details
   
Sequence Name 44-K118396-0022-544-E01C-57wj
GenBank Accession LN505336 [GenBank]
Sequencing primer gene specific primer, 57wj: 5'-TGAATCATATGTTGAAATCTAATCGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>44-K118396-0022-544-E01C-57wj
GGATTTAAGAAAAATCCTTTTACATATTCCCATGATGACACAAAATTAAAAATGTTACAA
CTTGATTAGAAAACTACAACAACGTATATTATATGCGTTTGTTGTATGTTTTTGTGCTTG
TAGACTACCTTCACGTCCTCTTATTCTTACTCCCGAACAGCAAGGACAAGTAAAACAAGA
TCCTAAAGAAGAGACCCCAAGCTAACGTAATCCATAAGCAATCCCACTTGCTCAATTGAG
TTATACCTTGCTGCATAAGTAAGTCAGGTCCTGTTCTCAAGCATGTGGACTCTGTTATCT
TCGTTCCTAAAGAGCCACTCAGTGTATCTAGGAGCTTAACCTTCATCACATGGGACACTT
CCGCCAAAAGCGTACCATCAAATACTTGAAATGGCTTCATGTCCGGGAAATCTACATGGA
TCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTT
CAATTCAAAAATGTAGATGTCCGCAGCGGTTATTAT
   
Sequence Name 41-K118394-0022-544-E01C-8409
GenBank Accession LN504254 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K118394-0022-544-E01C-8409
CCATTTCAGTATTTGATGGTACGCTTTTGGCGGAAGTGTCCCATGTGATGAAGGTTAAGC
TCCTAGATACACTGAGTGGCTCTTTAGGAACGAAGATAACAGAGTCCACATGCTTGAGAA
CAGGACCTGACTTACTTATGCAGCAAGGTATAACTCAATTGAGCAAGTGGGATTGCTTAT
GGATTACGTTAGCTTGGGGTCTCTTCTTTAGGATCTTGTTTTACTTGTCCTTGCTGTTCG
GGAGTAAGAATAAGAGGACGTGAAGGTAGTCTACAAGCACAAAAACATACAACAAACGCA
TATAATATACGTTGTTGTAGTTTTCTAATCAAGTTGTAACATTTTTAATTTTGTGTCATC
ATGGGAATATTGTAAAAGGATTTTTTCTTAAAATCCTTAGTATCATAATAATTACTCGAG
TACACGATTAGATTTCACATAATGATTC

Last Updated on 06.12.2018 16:11