Sequences from confirmation amplicon


Confirmation sequences for line 544F04 and hit At2g42200 are shown.
Line ID 544F04
Locus At2g42200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17587749
Primers used for PCR PG12 - 8474, show primer details
   
Sequence Name 56-K101746-0022-544-F04C-8409
GenBank Accession LN509053 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K101746-0022-544-F04C-8409
TTACAATTGAATATATCCTGCAAATGTAAATGGATAGGCAATGAGATCTCGAAATCTACA
TGGATATATTCATGGTCAATTGTATATTCATGAAGACCCGGATCCACCACCACCGTCCTC
GAAGTAGATCTTCTGGCCAAACATGAGCCCTCCACTGAAAGAGGATGACTCAGTGGAGGA
ACCACCCGACTCTGCCTGACCCGGACCATGACCCGGACCCGAGTTGGAACCCATCTCCAT
GTTGGTTTCCTCTTACTCAGACAGAAAGGGTTTTACTCATCATTCTCTCTCTCACACAAA
CCTAACTGTCTCGGGTTTAAAGAAAGGACCAAAGGAGAAGAAAGCAAAGAAAATTTCGGC
TTCCCTGATTTTGACTGTTTTGGGTTTAGAGAGAGAAACAGAACAGAAGGAAAAAAGAAA
GAGACGAGAGTGGTAATAGACAAAGACAGTGAGAGAGTAAGTAAAACGGTACGGACAAAC
AAAAACTGTTGTCTGTTGTTAATTACCACATAGCANAACGAGGAAGCTAT
   
Sequence Name 56-K101752-0022-544-F04C-PG12
GenBank Accession LN509054 [GenBank]
Sequencing primer gene specific primer, PG12: 5'-TATAGCTCCCTCGTTTTGCTATGT-3'
Sequence (A. th genome BLAST matches underlined) 
>56-K101752-0022-544-F04C-PG12
GTTTTTGTTTGTCCGTACCGTTTACTTACTCTCTCACTGTCTTTGTCTATTACCACTCTC
GTCTCTTTCTTTTTTCCTTCTGTTCTGTTTCTCTCTCTAAACCCAAAACAGTCAAAATCA
GGGAAGCCGAAATTTTCTTTGCTTTCTTCTCCTTTGGTCCTTTCTTTAAACCCGAGACAG
TTAGGTTTGTGTGAGAGAGAGAATGATGAGTAAAACCCTTTCTGTCTGAGTAAGAGGAAA
CCAACATGGAGATGGGTTCCAACTCGGGTCCGGGTCATGGTCCGGGTCAGGCAGAGTCGG
GTGGTTCCTCCACTGAGTCATCCTCTTTCAGTGGAGGGCTCATGTTTGGCCAGAAGATCT
ACTTCGAGGACGGTGGTGGTGGATCCGGGTCTTCATGAATATACNATTGACCATGAATAT
ATCCATGTAGATTTCGAGATCTCATTGCCTATCCATTTACATTTGCAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGC

Last Updated on 06.12.2018 16:11