Genotyping and primer details


Genotyping and primer details for 544F04 - At2g42200
Line ID 544F04
Locus At2g42200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17587749 of line 544F04 Chr2:17587749
   
Insertion allele amplicon 8474 - PG12
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PG12: 5'-TATAGCTCCCTCGTTTTGCTATGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17587310
   
wt amplicon (over InsPos) 18fg - PG12
wt amplicon start Chr2:17587310
wt amplicon end Chr2:17588117
wt amplicon size 808 bp
Locus specific primer PG12: 5'-TATAGCTCCCTCGTTTTGCTATGT-3'
2nd locus specific primer 18fg: 5'-ATGACACTGACAATCACAAAAAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11