Sequences from confirmation amplicon


Confirmation sequences for line 544G08 and hit At3g10740 are shown.
Line ID 544G08
Locus At3g10740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:3363233
Primers used for PCR BE57 - 8474, show primer details
   
Sequence Name 69-K028751-022-544-G08C-8409
GenBank Accession LN512115 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K028751-022-544-G08C-8409
GGAACCGGAAGAAACGGGGTTTTCTCTCGGCCATCATTTGGAAAAGATCGTTTCTAAAAC
CACGTCCCTGCAATCAACCAAAAAGATCGAGTATTTGATAGAGTTCTTCTTCCAATATTT
TCTCTATTAGCAATCTGTAATCTTCCATCTCAAGGCTCTACAGGGAATCAGAGAGAGCGA
AAAAAGAACCTTGTGAGTATCCACTGGCATGGCCGAGACTTGGTCAATCCATATTGACCC
TTTCTTGGTTGTTGTTAACTGAAGTCTTGCACTAGGATCGGTCGCTTTTGCCTCCAAAAG
AACCTCTTTTTTAATCCATTTCGAAACATCAGAAGCAGAAGCTCTACATTGAAACAAATG
AAAGCATACAATTGAAACTTTCACACACTGAAACAGCAAAATAAACATATTCAGACTTAA
CTGAAGAATCTGAAATTATTTCTCACATGATCTTTTCAGAAGCAAGAGTCCGTGATCCAT
TCGAGCTCGTCAACGACACA
   
Sequence Name 69-K028751-022-544-G08C-BE57
GenBank Accession LN512116 [GenBank]
Sequencing primer gene specific primer, BE57: 5'-GTGATATCGATTTGTCTGTGTCGT-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K028751-022-544-G08C-BE57
AAAAAATTTCAGTTTCTTCCAGTAAGTCGAATAGTTTATTTTGCGTTTCAGTGTGTGAAA
GTTTCAATTGTATGCTTTCATTTGTTTCAATGTAGAGCTTCTGCTTCTGATGTTTCGAAA
TGGATTAAAAAAGAGGTTCTTTTGGAGGCAAAAGCGACCGATCCTAGTGCAAGACTTCAG
TTAACAACAACCAAGAAAGGGTCAATATGGATTGACCAAGTCTCGGCCATGCCAGTGGAT
ACTCACAAGGTTCTTTTTTCGCTCTCTCTGATTCCCTGTAGAGCCTTGAGATGGAAGATT
ACAGATTGCTAATAGAGAAAATATTGGAAGAAGAACTCTATCAAATACTCGATCTTTTTG
GTTGATTGCAGGGACATGGTTTTAGAAACGATCTTTTCCAAATGATGGCCGATATAAAAC
CCCGTTTCATCCGGTTCCCAGGTACGTATTATTTAAGGCTAGTAGCATTTTTGTTTTTCC
AGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAG
TATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAA
TGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTAC
GATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTAT
TTCGACGTGTCTA

Last Updated on 06.12.2018 16:11