Sequences from confirmation amplicon


Confirmation sequences for line 545C04 and hit At1g09810 are shown.
Line ID 545C04
Locus At1g09810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3181250
Primers used for PCR JL55 - 8474, show primer details
   
Sequence Name 19-K101383-0022-545-C04C-8409
GenBank Accession LN494693 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K101383-0022-545-C04C-8409
CCATTTACATTGGAACAGAAGTAGCAATAGCAAAACACAAGCATCTCTTGTCAATCAAAC
CAAGTATCTTTCCATAAGAGGCTACTCTGGTTACAAGAAGAACACTGGCAATTCAACATG
AGATGACATTCGTCAATCTTTAATTCCTCGTCTTCTTTATTTTATTCATTCATGAATTGC
TTTAGTCAGTTCAGGAATCATTAGGGTTTTTTTTTCTTCCGTTGTTAGGGTTTGGTTTCT
ATTTTTTCTATTTCATATTCAGATGTCTTTAGGCCCACGGATTCATTTTCACGATCTCAA
TGATAAATCACTTTTCAGATACAGCGAGGAAATTAGGGTAAAGTAAACAAATCATGATCA
TTAGAAGAAGATGCACACAAATGTGTTCGGAATTTTCTCAGAAACTAAGCAAACTGTTCT
TTGCTCTTTCATAAGTTTTGGTTTTTGTCCCACATCGACAAGTAGAAGAGAACGTCTCCC
AAGTCTGATAATATATAAAGGAAGCTGGGTCTCACTTTCAAATCATACCTTTCTCGGCCT
TTTGGCTAAAATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATATGTGGGCCA
TCGGCCCACACGATATTAACTCTATTTTTAAAGGGAGAAAGC
   
Sequence Name 19-K101389-0022-545-C04C-JL55
GenBank Accession LN494694 [GenBank]
Sequencing primer gene specific primer, JL55: 5'-CTATCTTAGTGGGCTTTCTCCCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K101389-0022-545-C04C-JL55
GCCGAGGCCCCATATCAGATATTAAACTGATAAGAACAGATACTACCTTTAATCTTAGCC
AAAAGGCCGAGAAAGGTATGATTTGACTTTGAGACCCAGCTTCCTTTATATATTATCAGA
CTTGGGAGACGTTCTCTTCTACTTGTCGATGTGGGACAAAAACCAAAACTTATGAAAGAG
CAAAGAACAGTTTGCTTAGTTTCTGAGAAAATTCCGAACACATTTGTGTGCATCTTCTTC
TAATGATCATGATTTGTTTACTTTACCCTAATTTCCTCGCTGTATCTGAAAAGTGATTTA
TCATTGAGATCGTGAAAATGAATCCGTGGGCCTAAAGACATCTGAATATGAAATAGAAAA
AATAGAAACCAAACCCTAACAACGGATGAAAAAAAAACCCTAATGATTCCTGAACTGACT
AAAGCAATTCATGAATGAATAAAATAAAGAAGACGAGGAATTAAAGATTGACGAATGTCA
TCTCATGTTGAATTGCCAGTGTTCTTCTTGTAACCAGAGTAGCCTCTTATGGAAAGATAC
TTGGTTTGATTGACAAGAGATGCTTGTGTTTTGCTATTGCTACTTCTGTTTCCATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTANCAATTTTTTTT

Last Updated on 06.12.2018 16:11