SimpleSearch - Line and FST details
Line specific information
| Line ID | 545C04 |
| Vector Used | pAC161 |
| Line Availability | available as T3 set from NASC (N452252) |
| Segregation Analysis | 50:29:19 |
| Confirmed for Hit | At1g17600 At1g09810 |
| Parent of DUPLO pair | 2815 |
| Parent of pair(s) | none |
| Note |
Gene hit At1g17600
| Sequence (A. th genome BLAST matches underlined) | >27-K020968-022-545-C04-8409 NAGCCATTTACAATTGAATATATCCTGACAGTGTAATATGAAGACGTGAAATGATAAGTT GAGATTGAGTTATTCAATTAAAGTTTACTGCATCCTGACCCGTAGAATTTTGGAACTTTC TGAATATAATGATCTTGAATAATCAACTGGGAGTCCATATTAATTTATTGTGCCTAGACT TGCGTGATTTGACTTGTGAGTTATCACGTGTATGCTATTAGTTCTTGGCTGGAATGCATA ATCTTCATGTTTCTCTGGGCTTACAGGCATGTGAATTACATAGAGTCGAATCTGCAACTC ATTCCAGACCAACCGTTCCTGCCTCGTAGCCTACGGCTATTCCATTGGGATGCATTTCCA TTGANAGCCCTGCCTTCCGGTTCTGATCCATGTTTTCTTGATNAACTCAATCTGCGTCAC AGTGGTTTATAAAC |
| GenBank Accession | BX572274 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:6054921 - go to primer design |
| BLAST e Value | 2e-75 |
| Hit Clone Code (BAC ID) | F1L3 |
| Hit Gene Code | At1g17600 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | Disease resistance protein (TIR-NBS-LRR class) family |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
| Other FSTs Supporting this Hit | BX650388 [GenBank] |
Gene hit At1g09810
| Sequence (A. th genome BLAST matches underlined) | >27-K020629-022-545-C04-8409 GAAGAATCTTTCCATAAGAGGCTACTCTGGCTTCAAGAAGAACATTGGCAATTCAACATG ATTATGACATTCGTCAATCTTTAATTCCTCGTCTTCTTTATTTTATTCATTCATGAATTG CTTTATTCAGTTCAGGAATCGTTAGGGTTTTTTTTCCTTCCGCGGCGCGGGCTGGCCCCG CCGCCCCCGGGCGGTCGCGGGCTGTGAATTAAACAAATATTTTTAGTATCTTTTGATGTA GAAAAAACTTTCATG |
| GenBank Accession | BX547564 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr1:3181226 - go to primer design |
| BLAST e Value | 1e-55 |
| Hit Clone Code (BAC ID) | F21M12 |
| Hit Gene Code | At1g09810 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | evolutionarily conserved C-terminal region 11 |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
Gene hit F24J8
| Sequence (A. th genome BLAST matches underlined) | >27-K020660-022-545-C04-8409 AAATACTTTATGTACCACTTGATAAGATTTGATCGGTTCGGTTTATTCCGTCTCAGGCTA CTCCGGCCAGAACAATAACATTGGCGATTTATCTTGATATGACTATCTTCAATCTTTAAC TCCTCGTCTACTTTATTTTATTCTTTCTTGAATTGCTTAAATTAGATTAAGATTCATTAT GTATTTTTAATATTTTTTGGTGTTAAAGGAACTTTCATGCCTATGTGATCATCCCGGTCG TGAGGGAAAAAAATAGAAAGTATTTCCCATTTTTTTTTCAATCCCAAAATGTGAATGCCC CCACCCTTAATAATAAGACC |
| GenBank Accession | BX547565 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:7505253 - go to primer design |
| BLAST e Value | 6e-17 |
| Hit Clone Code (BAC ID) | F24J8 |
| Insertion Classification | Genome Hit |
| Confirmation Status | unknown |
Gene hit T22A6
| Sequence (A. th genome BLAST matches underlined) | >70-K022079-022-545-F09-8409 AACCATTTACATTGAATATATCCTGTGGCATCCTCCCTATAGTGAGACTATGGGATCCTC CCTATAGTGAGACTATAGAATCCTCCCGATACTAAGACTTGGCAAGTCTTGGCAATATAG TTCCACTCGACAATTTTATATTGTTTCACTAACTATCCATCTATAGACTATAATGCATAC ATTAAATATATTAATCTTAACAGAACAAAATTTTCAATTAATTTTGAATATATTATGCAC GAGTCGTTACCTATGGGATCCTCCCTATAGTGAGAAAAGACGATCCGCCCGTCACTCAAG ACCAATCGTCTCTGCCTCATAACCAGGGACCATCCTTATACAATACTAACGTTTCTAACA CAGCCTTACACATCTAAAACATTGACGATGAAAAAAAACACTACAAAAAACCACATAACG AAGAAGAAACATTAAGAACTCAAGACGCAAACATATAAAGGAGAGAAAATCGAA |
| GenBank Accession | BX650386 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr4:12598613 - go to primer design |
| BLAST e Value | 1e-64 |
| Hit Clone Code (BAC ID) | T22A6 |
| Insertion Classification | Genome Hit |
| Confirmation Status | failed |
|
Last Updated on 10.06.2021 13:37 |
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