Sequences from confirmation amplicon


Confirmation sequences for line 545C04 and hit At1g17600 are shown.
Line ID 545C04
Locus At1g17600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6054929
Primers used for PCR CW76 - 8474, show primer details
   
Sequence Name 27-K104153-0022-545-C04C-CW76
GenBank Accession LN498361 [GenBank]
Sequencing primer gene specific primer, CW76: 5'-GATCCCGTCACATCTAGTCTCTTC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K104153-0022-545-C04C-CW76
ATGGAAATAGTTGGAATTATGTTTCTCGCAAGGATTCTCTGTTTTCACACCATTGCTCTA
TCAAAAACACAAACACAAGATGCAGAATCATAGAAAAGATTACCGGTGTTCCACTCCAGA
GAGTTTCTAAATCACTGTGACGCAGATTGAGTTCAACAAGAAAACATGGATCAGAACCGG
AAGGCAGGGCTCTCAATGGAAATGCATCCCAATGGAATAGCCGTAGGCTACGAGGCAGGA
ACGGTTGGTCTGGAATGAGTTGCAGATTCGACTCTCTGTAATCTACATGCTTGTAAACCT
TGAGAAACTTGAGATTATGCATACGGCCAACAACACTGGCCTCCATGGATAACACACAGG
TCATATCACACGTGTGTAGGCACATGCATTCAGTTTGTTCTCCTCCCTGATGATAAGAAG
ACAAAAGAAACACAAAGCCAGAAACTCGTCAGGTCAGGATGCATGAAAATTTAAAAGAAT
AGATCAAAGATGATATAGATGCTTACGTCTCTGAAAGCCAGGGCAACGACATATTCAATT
GTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATAT
GGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 27-K104154-0022-545-C04C-8409
GenBank Accession LN498362 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K104154-0022-545-C04C-8409
CGGACATGAGCCTTTACAATTGAATATGTCGTTGCCCTGGCTTTCAGAGACGTAAGCATC
TATATCATCTTTGATCTATTCTTTTAAATTTTCATGCATCCTGACCTGACGAGTTTCTGG
CTTTGTGTTTCTTTTGTCTTCTTATCATCAGGGAGGAGAACAAACTGAATGCATGTGCCT
ACACACGTGTGATATGACCTGTGTGTTATCCATGGAGGCCAGTGTTGTTGGCCGTATGCA
TAATCTCAAGTTTCTCAAGGTTTACAAGCATGTAGATTACAGAGAGTCGAATCTGCAACT
CATTCCAGACCAACCGTTCCTGCCTCGTAGCCTACGGCTATTCCATTGGGATGCATTTCC
ATTGAGAGCCCTGCCTTCCGGTTCTGATCCATGTTTTCTTGTTGAACTCAATCTGCGTCA
CAGTGATTTAGAAACTCTCTGGAGTGGAACACCGGTAATCTTTTCTATGATTCTGCATCT
TGTGTTTGTGTTTTTGATAGAGCAATGGTGTGAAAACAGAGAATCCTTGCGAGAAACATA
ATTCCAACTATTTCCATGTGTTGTTGTATCTTGCTCAGATGTTGAAGAGTTTGAAGAGAC
TAGATGTGACGGGAT

Last Updated on 06.12.2018 16:11