Sequences from confirmation amplicon


Confirmation sequences for line 545D03 and hit At1g25510 are shown.
Line ID 545D03
Locus At1g25510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8959621
Primers used for PCR 29op - 8474, show primer details
   
Sequence Name 15-K114972-0022-545-D03C-29op
GenBank Accession LN493093 [GenBank]
Sequencing primer gene specific primer, 29op: 5'-GTGTCTTTACGAGGTTTCTTACGG-3'
Sequence (A. th genome BLAST matches underlined) 
>15-K114972-0022-545-D03C-29op
TCGGCTCAACTCTAGTTCAAAACGTCGCCGTTGGTTGTGGACACAGCAACGAAGGGTTGT
TCGTCGGCGCCGCCGGTTTACTTGGACTCGGCGGTGGTTTGTTAGCTCTTCCGTCGCAGC
TCAACACGACGTCGTTCTCGTACTGTCTCGTTGACCGTGACTCAGACTCGGCTTCGACCG
TGGATTTCGGGACTTCTCTTTCTCCTGACGCCGTCGTGGCTCCGTTGCTACGTAACCACC
AGCTCGACACGTTTTACTACCTAGGCCTCACCGGAATAAGCGTCGGCGGCGAGTTGCTTC
AGATTCCTCAGTCTTCTTTCGAGATGGACGAGTCAGGCAGCGGTGGGATCATCATCGACT
CAGGAACCGCCGTGACTCGTCTCCAGACCGAAATCTATAACTCGCTTAGAGACTCGTTCG
TGAAAGGAACGTTGGACTTGGAAAAAGCAGCCGGGGTGGCGATGTTTGACACGTGTATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCG
   
Sequence Name 06-K114971-0022-545-D03C-8409
GenBank Accession LN488930 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K114971-0022-545-D03C-8409
GCATTTCATTGATATACCGTGTCAACATCGCCACCCCGGCTGCTTTTTCCAGTCCAACGT
TCCTTTCACGAACGAGTCTCTAAGCGAGTTATAGATTTCGGTCTGGAGACGAGTCACGGC
GGTTCCTGAGTCGATGATGATCCCACCGCTGCCTGACTCGTCCATCTCGAAAGAAGACTG
AGGAATCTGAAGCAACTCGCCGCCGACGCTTATTCCGGTGAGGCCTAGGTAGTAAAACGT
GTCGAGCTGGTGGTTACGTAGCAACGGAGCCACGACGGCGTCAGGAGAAAGAGAAGTCCC
GAAATCCACGGTCGAAGCCGAGTCTGAGTCACGGTCAACGAGACAGTACGAGAACGACGT
CGTGTTGAGCTGCGACGGAAGAGCTAACAAACCACCGCCGAGTCCAAGTAAACCGGCGGC
GCCGACGAACAACCCTTCGTTGCTGTGTCCACAACCAACGGCGACGTTTTGAACTAGAGT
TGAGCCGATTGTGAGAGTCTCGGTGGCGAAATCTCCAACGGTGTAAGAACCATCACCGTA
AGAAACCTCTAANAGACA

Last Updated on 06.12.2018 16:11