Sequences from confirmation amplicon


Confirmation sequences for line 545D05 and hit At5g41315 are shown.
Line ID 545D05
Locus At5g41315 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16530750
Primers used for PCR MJ23 - 8474, show primer details
   
Sequence Name 49-K024605-022-545-D05C-8409
GenBank Accession LN506704 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K024605-022-545-D05C-8409
GTAGATTTCCCGGACATGAAGCCATTTACAATTGAATATATACTCATTGCTGCAATGCAT
TCTAAGCTTAATTTTTCCACATTTTTTCTTCCTAACATATTCATAATTATTGAAAGTTAA
TTAGCCGTGTTCATAAAGACCAAAATGAAACGAATTTTTTAATTAATCCGGACAGAAACA
AACTTTTGTGATGATAAATAAACCGAACTAAACTCGAATCGAACACAAATATACTGCAAC
CAAACCGAATTAATAAAATTTATTAGATGTAGAATAGAGTTAAACATACTAATGCATATA
TATACACATTATTATTTAAAATTAATTATGTTTTAACTTTTTCTTTTGTAATCTGAAACC
GACCAAATCCGGACATTAAATTTTGAAACCAAACTGAAACTAAAAAATTGGTTCTAGCTC
ATACTTTTTTCCTGCAGAACCCTGAAATCTAACAAACCTAAACTGAACCATAAAAGTCGA
AATCCTTGGACCTACTAGTATTAAAAGTATATTTTGGCACGTTGTTTAAAACTGGCAAAG
ATCAACACGATCATGACACATAATAAATGTCAATGCTGATAGAGGAAAAGACGGAATTAC
CCTTCACCAATGTT
   
Sequence Name 50-K024605-022-545-D05C-MJ23
GenBank Accession LN507045 [GenBank]
Sequencing primer gene specific primer, MJ23: 5'-TATGTCTTTCGTCTTCAACATTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K024605-022-545-D05C-MJ23
GGGTAATTCCGTCTTTTCCTCTATCAGCATTGACATTTATTATGTGTCATGATCGTGTTG
ATCTTTGCCAGTTTTAAACAACGTGCCAAAATATACTTTTAATACTAGTAGGTCCAAGGA
TTTCGACTTTTATGGTTCAGTTTAGGTTTGTTAGATTTCAGGGTTCTGCAGGAAAAAAGT
ATGAGCTAGAACCAATTTTTTAGTTTCAGTTTGGTTTCAAAATTTAATGTCCGGATTTGG
TCGGTTTCAGATTACAAAAGAAAAAGTTAAAACATAATTAATTTTAAATAATAATGTGTA
TATATATGCATTAGTATGTTTAACTCTATTCTACATCTAATAAATTTTATTAATTCGGTT
TGGTTGCAGTATATTTGTGTTCGATTCGAGTTTAGTTCGGTTTATTTATCATCACAAAAG
TTTGTTTCTGTCCGGATTAATTAAAAAATTCGTTTCATTTTGGTCTTTATGAACACGGCT
AATTAACTTTCAATAATTATGAATATGTTAGGAAGAAAAAATGTGGAAAAATTAAGCTTA
GAATGCATTGCAGCAATGAGTATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAAGTATGATGGTCAATATGGAGAAAAAGAAAGAG

Last Updated on 06.12.2018 16:11