Genotyping and primer details


Genotyping and primer details for 545D05 - At5g41315
Line ID 545D05
Locus At5g41315 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16530750 of line 545D05 Chr5:16530750
   
Insertion allele amplicon 8474 - MJ23
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer MJ23: 5'-TATGTCTTTCGTCTTCAACATTGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16530172
   
wt amplicon (over InsPos) 17rd - MJ23
wt amplicon start Chr5:16530172
wt amplicon end Chr5:16531079
wt amplicon size 908 bp
Locus specific primer MJ23: 5'-TATGTCTTTCGTCTTCAACATTGG-3'
2nd locus specific primer 17rd: 5'-CATTGGAAGCAGAAAACACAAATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11