Sequences from confirmation amplicon


Confirmation sequences for line 545G01 and hit At5g42170 are shown.
Line ID 545G01
Locus At5g42170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:16850434
Primers used for PCR 12ib - 8474, show primer details
   
Sequence Name 31-K112562-0022-545-G01C-12ib
GenBank Accession LN500229 [GenBank]
Sequencing primer gene specific primer, 12ib: 5'-AAATCCAAAGCTCAAAAATGAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>31-K112562-0022-545-G01C-12ib
TTCTGGTACGATCCGTTAACGCAAAACTAGTGGTATTTTTAATATCGTTTAAACTTTCTT
AATAAATTGGAGTTTTGATTCTTATTTTTTAGTCATCTTTTTATTGATATGTTTTTTTTT
GTTCTGCGATGCTCCAGAAAGTGGTATCGTTATCAGATCAACTAAAAAATTTCCAAGAAT
ATAAAAATAAATTAAAGGTGATTGTTGGCGAAGAGAAAGCTAATTTCTTGGTGAAAAATA
GTCTTTATCTTGTGGTAGCCAGCAGTAATGATATTGCTCATACCTATACGGCACGCAATT
GAATATATTTCAGCAGTAAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCACGGTTATT
   
Sequence Name 07-K112561-0022-545-G01C-8409
GenBank Accession LN489343 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K112561-0022-545-G01C-8409
TCCTTACTGCTGAAATATATTCATTGCGTGCCGTATAGGTATGAGCAATATCATTACTGC
TGGCTACCACAAGATAAAGACTATTTTTCACCAAGAAATTAGCTTTCTCTTCGCCAACAA
TCACCTTTAATTTATTTTTATATTCTTGGAAATTTTTTAGTTGATCTGATAACGATACCA
CTTTCTGGAGCATCGCAGAACAAAAAAAAACATATCAATAAAAAGATGACTAAAAAATAA
GAATCAAAACTCCAATTTATTAAGAAAGTTTAAACGATATTAAAAATACCACTAGTTTTG
CTGTTAACGGATCGTAACCAGAACCACCTGAAGCAAAATTCACACCCTTGAGAAGATCTT
CATTTTTGAGCTTTGGAT

Last Updated on 06.12.2018 16:11