Sequences from confirmation amplicon


Confirmation sequences for line 546A07 and hit At3g09370 are shown.
Line ID 546A07
Locus At3g09370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2880238
Primers used for PCR GE51 - 8474, show primer details
   
Sequence Name 77-K100941-0022-546-A07C-8409
GenBank Accession LN513513 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K100941-0022-546-A07C-8409
CGGACTGAGCCATTTACAATTGAAGCCATCATACTCAATACTAACCTCTTCCGGAGTCCA
CCCACCTTTGGCTCGCCTTATAGGGCCACTCGTCCTCCTGCATACCAAACACTATCAACA
TCAATCTCTTGTAGAAAAGATGCAAATTCGCAAACGAGTGCTGCGGAGGAGATACAAAAC
AAGGAAGAAGAAGAAGGAGACGAACTAACCCGAGGTACCTATGAGTAGGTGATACTGTGG
CAGGACTTGCAATTGTAGGTGACTTGTGGGAACTACCAGCACTGCCTTCAGATACAGAAG
AGCACGAAGCAGGTGTTGATTGCTTATTTTCTACACACTGATCCTCATTNTTCACTTC
   
Sequence Name 77-K100949-0022-546-A07C-GE51
GenBank Accession LN513514 [GenBank]
Sequencing primer gene specific primer, GE51: 5'-TGAAGTGAAAATTGAGGATCAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>77-K100949-0022-546-A07C-GE51
CTCTTCTGTATCTGAAGGCAGTGCTGGTAGTTCCCACAAGTCACCTACAATTGCAAGTCC
TGCCACAGTATCACCTACTCATAGGTACCTCGGGTTAGTTCGTCTCCTTCTTCTTCTTCC
TTGTTTTGTATCTCCTCCGCAGCACTCGTTTGCGAATTTGCATCTTTTCTACAAGAGATT
GATGTTGATAGTGTTTGGTATGCAGGAGGACGAGTGGCCCTATAAGGCGAGCCAAAGGTG
GGTGGACTCCGGAAGAGGTTAGTATTGAGTATGATGGCTTCAATTGTAAATGGCTTCATG
TCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGA
GTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC

Last Updated on 06.12.2018 16:11