Genotyping and primer details


Genotyping and primer details for 546A07 - At3g09370
Line ID 546A07
Locus At3g09370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:2880238 of line 546A07 Chr3:2880238
   
Insertion allele amplicon 8474 - GE51
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GE51: 5'-TGAAGTGAAAATTGAGGATCAGTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:2879910
   
wt amplicon (over InsPos) 20si - GE51
wt amplicon start Chr3:2879910
wt amplicon end Chr3:2880612
wt amplicon size 703 bp
Locus specific primer GE51: 5'-TGAAGTGAAAATTGAGGATCAGTG-3'
2nd locus specific primer 20si: 5'-CGGAAACAAACGTAAAAGAAAGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11