Sequences from confirmation amplicon


Confirmation sequences for line 546D06 and hit At4g10720 are shown.
Line ID 546D06
Locus At4g10720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:6608664
Primers used for PCR 17qt - 8474, show primer details
   
Sequence Name 34-K115781-0022-546-D06C-17qt
GenBank Accession LN501543 [GenBank]
Sequencing primer gene specific primer, 17qt: 5'-TGACTCATCACAACAGTACCAACA-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K115781-0022-546-D06C-17qt
CACCTCCTGGTGGCTGCAAGCTGTTTGATAAGTAGCTGTGATTATCAGCGCGGCTATCAC
TAGAAGCGCGCTGCGTGTTCCTTCAGAAGTCTGGTTCCTATACCGGGCTGTTTGCGTGAA
CAAATGCTCGGTGAAACTAATCGGAGATCTCAAGATTTCCGACACTTTCTTGGATTTTGG
TAGAGAGTTTCCACTCTTACCTCCCCATTTTCGGATTATGTTTTCGATATTACTGTTTGC
GTGATGATCTCTCTGGTTATGTAAGATATCTAGGGCGGTTAAACCTGTCCGGTTATCGAT
TGAATATGATGGAAACCCATATGTTAATCAGTGCTGTCCGGTTTAAACTATCAGTGTTTT
AAACCCATATGTCCGGCTAAACATTTACTGATTGAATATATAGTGGTCAGGATATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 10-K115780-0022-546-D06C-8409
GenBank Accession LN490800 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K115780-0022-546-D06C-8409
ATTTACATTGAAATATCCTGACCACTATATATTCAATCAGTAAATGTTTAGCCGGACAGT
ATGGGTTAAAACACTGATAGTTTAAACCGGACAGCACTGATTAACATATGGGTTTCCATC
ATATTCAATCGATAACCGGACAGGTTTAACCGCCCTAGATATCTTACATAACCAGAGAGA
TCATCACGCAAACAGTAATATCGAAAACATAATCCGAAAATGGGGAGGTAAGAGTGGAAA
CTCTCTACCAAAATCCAAGAAAGTGTCGGAAATCTTGAGATCTCCGATTAGTTTCACCGA
GCATTTGTTCACGCAAACAGCCCGGTATAGGAACCAGACTTCTGAAGGAACACGCAGCGC
GCTTCTAGTGATAGCCGCGCTGATAATCACAGCTACTTATCAAACAGCTTTGCAGCCACC
AGGAGGTGTATATCAAGAAAATGCAGCAGAAGAAAGTAAAAAGAGTGTTGGTACT

Last Updated on 06.12.2018 16:11