Sequences from confirmation amplicon


Confirmation sequences for line 546G06 and hit At4g17410 are shown.
Line ID 546G06
Locus At4g17410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9718391
Primers used for PCR 17jr - 8474, show primer details
   
Sequence Name 43-K113214-0022-546-G06C-8409
GenBank Accession LN504900 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K113214-0022-546-G06C-8409
CATTTACATGAGAGATGCTGCACTGGCAAGCAAATGCTGCCTCAAGAGCTACTGTGACAA
GTGTTAGTTCTCTTGTCAAATTGCCAATACTTCTGATTCCTTTTGCTGTTCCACAAACGC
CTTTTTATGTAATCATTATCTACAATTCAGGTATTAGAGATCACATCATTGCAAAGTCAA
TGTGTGTTTGTGGAGCAACTCATGTCCTAGCTGATGATCTTCTACCTAACAAAACCCTAA
GGGATACTATAAACCGGATATTGGAGTCTGGTAACAGTAGTGCTGAGAACGCTGGCAGCA
TGTGTCAAGTCCAAGGTATTTCATACTCTCATTCGCAGTCTTTTATATTTGTGTGTACCA
TCATTGGTAAACTAATGAGTATTAAATCTAGATATGGAGTCTGTACGGTGTCCACCTCCC
AAGGCTCTATCTCCTACTACATCTGCTGCATCTGGAGGCGAAAAGAAACCAGCTCCTAGT
AACAACAATGAGACTTCAACTCTAAAGCCGTCAATAGAAATAGCAGAGATCACAAGTGCT
TGGGCGTCTGCAGAAATAGTTAAAGTGGAAAGCCCAGTTGA
   
Sequence Name 43-K113215-0022-546-G06C-17jr
GenBank Accession LN504901 [GenBank]
Sequencing primer gene specific primer, 17jr: 5'-TCAACTGGCTTTTCCACTTTAACT-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K113215-0022-546-G06C-17jr
TTCTATTGACGGCTTTAGAGTTGAAGTCTCATTGTTGTTACTAGGAGCTGGTTTCTTTTC
GCCTCCAGATGCAGCAGATGTAGTAGGAGATAGAGCCTTGGGAGGTGGACACCGTACAGA
CTCCATATCTAGATTTAATACTCATTAGTTTACCAATGATGGTACACACAAATATAAAAG
ACTGCGAATGAGAGTATGAAATACCTTGGACTTGACACATGCTGCCAGCGTTCTCAGCAC
TACTGTTACCAGACTCCAATATCCGGTTTATAGTATCCCTTAGGGTTTTGTTAGGTAGAA
GATCATCAGCTAGGACATGAGTTGCTCCACAAACACACATTGACTTTGCAATGATGTGAT
CTCTAATACCTGAATTGTAGATAATGATTACATAAAAAGGCGTTTGTGGAACAGCAAAAG
GAATCAGAAGTATTGGCAATTTGACAAGAGAACTAACACTTGTCACAGTAGCTCTTGAGG
CAGCATTTGCTTGCCAGTGCAGCATCTCTCATGTAAATGGCTTCATGTCCGGGAAATCTA
CATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATT
TTTTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11