Sequences from confirmation amplicon


Confirmation sequences for line 548D04 and hit At5g18760 are shown.
Line ID 548D04
Locus At5g18760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6259203
Primers used for PCR 29fr - 8474, show primer details
   
Sequence Name 66-K111632-0022-548-D04C-29fr
GenBank Accession LN511640 [GenBank]
Sequencing primer gene specific primer, 29fr: 5'-TGCAAAAAGCATTGCATAGATAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K111632-0022-548-D04C-29fr
TTTACATGAGAACTTTTTCCATTGTAGGTATGTGATTATGTGTTTTAAGTAATTCAAAGC
CTAAAACATACGGTTTAGTATTACTTACTGTAGCATATAATAGATGAACAAGCAATGCCA
CTAGTGCAAACTCAAGAGCTGCGTATGTCCATATGTATTCTCTGATCGCTGCAAGTCCGG
AAAGTAACAGTTAACAGTTTTACATAATTGTGTTGGGGGAAGAACAATATAGATTGGAAA
TTTTTCTTGAGGGAGTGGTATTATACCGAGGATGACGGCAAAAGTTGAGGCTAAGAGTGC
AAGGGTGAATGAAAATGGCGCTGCAACAAATATTGCCTGTGAATTCAAGTCACGAATCTA
CAGAATAGATAAGATTCCCTGTTATGTTGTCTTCGCAATGAAAGGTAAGATCTTTGTAAT
GAAAGATGGGATATTTGTTATGAAATACCGGAATGTATGTGTTTTTGAATACTCGGGGTA
AGAAAAAGGAAGCATTACCAGTAAATGTTCAAGGAAGAAGAAGTAGCAGACGGTGCTGAT
GAGTAAATCGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCACGGTTATT
   
Sequence Name 50-K111631-0022-548-D04C-8409
GenBank Accession LN507212 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K111631-0022-548-D04C-8409
GCCATTTACGATTACTCATCAGCACCGTCTGCTACTTCTTCTTCCTTGAACATTTACTGG
TAATGCTTCCTTTTTCTTACCCCGAGTATTCAAAAACACATACATTCCGGTATTTCATAA
CAAATATCCCATCTTTCATTACAAAGATCTTACCTTTCATTGCGAAGACAACATAACAGG
GAATCTTATCTATTCTGTAGATTCGTGACTTGAATTCACAGGCAATATTTGTTGCAGCGC
CATTTTCATTCACCCTTGCACTCTTAGCCTCAACTTTTGCCGTCATCCTCGGTATAATAC
CACTCCCTCAAGAAAAATTTCCAATCTATATTGTTCTTCCCCCAACACAATTATGTAAAA
CTGTTAACTGTTACTTTCCGGACTTGCAGCGATCAGAGAATACATATGGACATACGCAGC
TCTTGAGTTTGCACTAGTGGCATTGCTTGTTCATCTATTATATGCTACAGTAAGTAATAC
TAAACCGTATGTTTTAGGCTTTGAATTACTTAAAACACATAATCACATACCTACAATTGG
AAAAAGTTCTCATGTAAATATCTTTTGTTGCAGCTTCGTGTGCCGGTTATCTATGCAATG
CTTTTTGC

Last Updated on 06.12.2018 16:11