Genotyping and primer details


Genotyping and primer details for 548D04 - At5g18760
Line ID 548D04
Locus At5g18760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:6259203 of line 548D04 Chr5:6259203
   
Insertion allele amplicon 8474 - 29fr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 29fr: 5'-TGCAAAAAGCATTGCATAGATAAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:6258607
   
wt amplicon (over InsPos) 04gs - 29fr
wt amplicon start Chr5:6258607
wt amplicon end Chr5:6259740
wt amplicon size 1134 bp
Locus specific primer 29fr: 5'-TGCAAAAAGCATTGCATAGATAAC-3'
2nd locus specific primer 04gs: 5'-TTCACGAGAAGCTTTATCACTTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11