Sequences from confirmation amplicon


Confirmation sequences for line 549G09 and hit At1g09840 are shown.
Line ID 549G09
Locus At1g09840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:3198518
Primers used for PCR JC10 - 8474, show primer details
   
Sequence Name 23-K027364-022-549-G09C-8409
GenBank Accession LN496418 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K027364-022-549-G09C-8409
CATTTACAATTGAATATATCCTGTCACCATTAACAGAAACTTATCAAAAATGTTTGAAGA
AAACCCANGCGGATTATCAATCTGGCATGCATTTACAAATATTATGAAGCAAAAACTACA
AAAAAGATTAAAAAAGGTTCAAATGTAGGAAATAGTTACCTGATAGGTGTAGAGTTTAAC
ATATATCAATGGCATCAGCTGGTTTGTCCTACTATAACTTCTTGCAACACGATTGACGGT
CTCAGGCACAAATTCAAGGACAAGATTCAAATAAACCTCTTCGTTGTCTGTTCTTGAGAA
AAAGCTATGCTTTAAAGCAACGGCATTGGGATGGTCTAGCATCTGCATTATTTGTAGCTC
CCTGTTCTTGTAGCGTTTGTCTTGTAGAACCTTCTTGATGGCAACAACCTCCCCAGTTTC
CCTACATTTAGCCTACCAAAAGGAAACATAAATGTGCAGGGGAATGGAGCCAAATCAATA
GAACGAACATCATGGGTCTTATGTTAAATTAGCTCACTTGGAAAACCATGCCAAAGGAAC
CAGTACCAACAACATGTTCTGATATGTAACTGACTGTCTGCCAATCCAAAAAAAATCTAT
ATGAGAAACAGTTAGATAAACTACATTACCAACTAACACATACAATTATCCATAACCATC
CAACCTGTCTTGATTGACCATTTCGACCACGGAGTGTGGTTCTAATCACATGACCAGGCT
CAGCACCAGC
   
Sequence Name 24-K027364-022-549-G09C-JC10
GenBank Accession LN496855 [GenBank]
Sequencing primer gene specific primer, JC10: 5'-GTGAACCAGATATTATTGATGGGG-3'
Sequence (A. th genome BLAST matches underlined) 
>24-K027364-022-549-G09C-JC10
GACACACTCCGNTGGTCGAAATGGTCAATCAAGACAGGTTGGATGGTTATGGATAATTGT
ATGTGTTAGTTGGTAATGTAGTTTATCTAACTGTTTCTCATATAGATTTTTTTTGGATTG
GCAGACAGTCAGTTACATATCAGAACATGTTGTTGGTACTGGTTCCTTTGGCATGGTTTT
CCAAGTGAGCTAATTTAACATAAGACCCATGATGTTCGTTCTATTGATTTGGCTCCATTC
CCCTGCACATTTATGTTTCCTTTTGGTAGGCTAAATGTAGGGAAACTGGGGAGGTTGTTG
CCATCAAGAAGGTTCTACAAGACAAACGCTACAAGAACAGGGAGCTACAAATAATGCAGA
TGCTAGACCATCCCAATGCCGTTGCTTTAAAGCATAGCTTTTTCTCAAGAACAGACAACG
AAGAGGTTTATTTGAATCTTGTCCTTGAATTTGTGCCTGAGACCGTCAATCGTGTTGCAA
GAAGTTATAGTAGGACAAACCAGCTGATGCCATTGATATATGTTAAACTCTACACCTATC
AGGTAACTATTTCCTACATTTGAACCTTTTTTAATCTTTTTTGTAGTTTTTGCTTCATAA
TATTTGTAAATGCATGCCAGATTGATAATCCGCTGGGTTTTCTTCAAACATTTTTGATAA
GTTTCTGTTAATGGTGACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCT
ACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAAT
TTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTT
GATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTC
TAAT

Last Updated on 06.12.2018 16:11