Sequences from confirmation amplicon


Confirmation sequences for line 550E07 and hit At4g39290 are shown.
Line ID 550E07
Locus At4g39290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:18285314
Primers used for PCR 31qz - 8474, show primer details
   
Sequence Name 74-K115869-0022-550-E07C-31qz
GenBank Accession LN513132 [GenBank]
Sequencing primer gene specific primer, 31qz: 5'-TGCAGATTCCTAACGAAGAGGTAT-3'
Sequence (A. th genome BLAST matches underlined) 
>74-K115869-0022-550-E07C-31qz
AGGTATAAGAACTATCCCGTAAGTTCATTAGAAAATAATCGTGCAACTTTCATGACTTAC
GAGATACACAAAGGTAGATGGAGAGAGCCACACTTATCGTTAAGTCATGGATTTCATTTC
TCTAATTGTGTGATAGAGAACGTGTTCTACCGTTATAGTTACGAGATGCTCCAATGGTAT
GACTCATGTAGAAAAATATGGAAAAATTTGAAAGGATTCGTGCGCAGGTCGATTATGAAT
CCTCGTGGTGAAGGTGTTAAAATGGTGAATTATGGAGGAAACATAGTTCTTTTGTGGGAG
GAGTGCGTGACTATTAAGAAGAAACTAATCTGGTGTGAAGAAGTTGTTATTGAACATCAG
GATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATG
TAGATGTCCACACGGTTAT
   
Sequence Name 33-K115867-0022-550-E07C-8409
GenBank Accession LN501131 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>33-K115867-0022-550-E07C-8409
CATTTCATTGAATATATCCTGATGTTCATAACAACTTCTTCACACCAGATTAGTTTCTTC
TTAATAGTCACGCACTCCTCCCACAAAAGAACTATGTTTCCTCCATAATTCACCATTTTA
ACACCTTCACCACGAGGATTCATAATCGACCTGCGCACGAATCCTTTCAAATTTTTCCAT
ATTTTTCTACATGAGTCATACCATTGGAGCATCTCGTAACTATAACGGTAGAACACGTTC
TCTATCACACAATTAGAGAAATGAAATCCATGACTTAACGATAAGTGTGGCTCTCTCCAT
CTACCTTTGTGTATCTCGTAAGTCATGAAAGTTGCACGATTATTTTCTAATGAACTTACG
TGGATAGCTTCTTTATACCCTACGATTTTGTAATTAAAACCTCTGCATACCTCTTCGTTG
AAAATCTGC

Last Updated on 06.12.2018 16:11