Sequences from confirmation amplicon


Confirmation sequences for line 550F01 and hit At1g09155 are shown.
Line ID 550F01
Locus At1g09155 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:2950806
Primers used for PCR 36sa - 8474, show primer details
   
Sequence Name 52-K116321-0022-550-F01C-36sa
GenBank Accession LN507925 [GenBank]
Sequencing primer gene specific primer, 36sa: 5'-GATTTTTCCGATGATTTCTAACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K116321-0022-550-F01C-36sa
CTATAATCAAATATTGAAACAAAATTATTGCTTACACTGTTACACCAATGATATGATTAA
ATTAATAACGACCTTGAATCTGATCGTGGGCTCCAAGACCAGTAATGTCTTTGATCGCTC
CAAGTTATGGAAAGATCTCTTGACGACAAAATATACGATATTTTCCCTGAAAGCTTCTCG
ATCTTGAAAATCTGAAATGAATTTTAACATTAAACCAAGAATTGGTTCACATGCAGAGAC
AGAGATAAAGATCACAGACCTTTCTGCCATTATCGATGAGAATTGATTCACAGAGACATC
GATAAAGCTCCTTTTTAGAAGAGAAAATTCGGTGAGGATCAGTCGATCTAGAGATGACGT
GGCAGTAATCGGTCGGTAAAAACTTCTCCCACACAAAGTCAGAGTCTCCGGCGACTCGGA
AAACTGATGAGACAGCCGCCGACGAGATTGTGTCAGCCGGAGTTGTAAATTGTAAATGGC
TTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAA
GAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 20-K116320-0022-550-F01C-8409
GenBank Accession LN495366 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K116320-0022-550-F01C-8409
TCATTTACAACTCCGGCTGACACAATCTCGTCGGCGGCTGTCTCATCAGTTTTCCGAGTC
GCCGGAGACTCTGACTTTGTGTGGGAGAAGTTTTTACCGACCGATTACTGCCACGTCATC
TCTAGATCGACTGATCCTCACCGAATTTTCTCTTCTAAAAAGGAGCTTTATCGATGTCTC
TGTGAATCAATTCTCATCGATAATGGCAGAAAGGTCTGTGATCTTTATCTCTGTCTCTGC
ATGTGAACCAATTCTTGGTTTAATGTTAAAATTCATTTCAGATTTTCAAGATCGAGAAGC
TTTCAGGGAAAATATCGTATATTTTGTCGTCAAGAGATCTTTCCATAACTTGGAGCGATC
AAAGACATTACTGGTCTTGGAGCCCACGATCAGATTCAAGGTCGTTATTAATTTAATCAT
ATCATTGGTGTAACAGTGTAAGCAATAATTTTTGTTTCAATATTTTGATTATAGGTTTTC
CGAAGGAGTCCAACTTATAATGACAGATTGGTTAGAAATCCGGAAAAAA

Last Updated on 06.12.2018 16:11