Sequences from confirmation amplicon


Confirmation sequences for line 551F03 and hit At2g21520 are shown.
Line ID 551F03
Locus At2g21520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9216568
Primers used for PCR PD17 - 8474, show primer details
   
Sequence Name 22-K109778-0022-551-F03C-PD17
GenBank Accession LN496154 [GenBank]
Sequencing primer gene specific primer, PD17: 5'-AATTTGTTTCCCCTTTCTCTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K109778-0022-551-F03C-PD17
TGTAAATGTGAGAAGTAGATAGATTCTCAACAAGAAAACATGAAAGGTTTTTTTTTTTTT
TGGGANCCGGGGGNCCCCCCCCCTTCAAAAAATTTGGGGAAAAACCCCAAAAACCCCACT
TTTTTTTCCATTTTTTTCCAAAGGGATTTTAACTGGGCGGGCCCCTTGGGCTTTTTTGCC
AAACCTTGTTAAAAGTCCCCCTTCTTTTTGGCCGGTTTTTTGTTTGGGGTTAATCTTTTC
CGGG
   
Sequence Name 06-K109777-0022-551-F03C-8409
GenBank Accession LN488857 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K109777-0022-551-F03C-8409
TCGGACATGACCATTTACAATTGAATATATCCTGTTATAACTATTTGTTTAGCTTTCTCC
CATCAAATTTCCTCGCTTTCAAGAACCTCAACATCATATGATAATCATCGTGTCTATCAG
GAAGCAACTCGTCCATTAACAACGACTGTCGAAACGCATCAACAGCTTGTAGCTCCTCGA
CATCCCTCACATCCTCAATGGATACAGAACTCACTCGACCATCACTTTTTCTTCTTCCGC
TCTTTTTCTTAAGAGAATGCTTGAACTTAGTAGAGGCATTAATGGCTTTCTTTTTCAAGG
AACCGATTCTTGTTCTCCTCTCATCCTCTGAATTCTCAAAATCTGACTTTCTTTCCTTTT
TCTCATCACTGCTCAAGAATCCCTCAAAACCTGTAGTGAAAGCTGTTTTGATTTGATGAG
ACACATTAAAAATTATCTAAAGCAATACACATTATTTAACCAGCTCAACAACATATATTG
AAGACACGAGAAAGCAAAGAGACACAATTTTTAGATTCTCATTATTATATTAGACACAAT
TCCAAGTTTAAGCTACAGTAAACCTAGAAAAAAGCTAACCCAGAAGCAAGAACATTACAA
AGAAGATTGAGATCTCTTACATGGTCTTGCAAATCGATCAAGTGGACCTGACATGTTAAA
TTCCTTTCGACTAAATATCGAAAAAATATTGTGATTCTTTGGATCTTTCCCAAATCTTTC
GAGAAGATGGCTGATCCACGAATCCAAAAAAAAAAAAAAAAAAACTTTTT

Last Updated on 06.12.2018 16:11