Sequences from confirmation amplicon


Confirmation sequences for line 553B10 and hit At3g55720 are shown.
Line ID 553B10
Locus At3g55720 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20681121
Primers used for PCR 46jd - 8474, show primer details
   
Sequence Name 80-K113077-0022-553-B10C-46jd
GenBank Accession LN513967 [GenBank]
Sequencing primer gene specific primer, 46jd: 5'-GGTTCGAGTCAATGGAGTTTAGAG-3'
Sequence (A. th genome BLAST matches underlined) 
>80-K113077-0022-553-B10C-46jd
TGGTACGTGGATTGGAGACAAGCCCTTGGCTCGCACATTCTCATGTATTCTATGAACCTT
CTGGACCTCTTAGAAGGTTCCTTCAAGGGCTTGATCCTAAAACCACAGCAAATTTATTTG
CGGGATCCGTTTGCGTGGGTGAAAAGGCGGTTAACAACGAAGAATGCTTTGTGCTTAAGC
TCGAAACGCAGCCTTCGGGACTTAAATCTAGAAGCAAGAGCGGTATGGAGACAGTGAAGC
ATACCGTGTGGGGATGTTTTGGTCAAAGAACAGGACTTTTGGTTCAATTAGAGGATACTT
ATCTAGTACGGATCAAGACCGGTCTGGAAGATGAAGATATGGTCTTGTGGGAAACGACGT
CTGAGACATTGATCCAAGATTATAAGAGCATTGATGGGATTCAGATCGCTCACAGAGGAA
AAACACGTGTCTCATTGTTGAGGCTTGATGAGAGCTTAGAGAGCCATTCAAAGACTACAA
TGGAAGAGAGTTGTGAATATACAATATATCCGATTATTCTAATATACAATTGTTTCTTTC
TTTTGAATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGC
   
Sequence Name 53-K113075-0022-553-B10C-8409
GenBank Accession LN508208 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K113075-0022-553-B10C-8409
CATTTCATTGAATTATTCAAAGAAAGAAACAATTGTATATTAGAATAATCGGATATATTG
TATATTCACAACTCTCTTCCATTGTAGTCTTTGAATGGCTCTCTAAGCTCTCATCAAGCC
TCAACAATGAGACACGTGTTTTTCCTCTGTGAGCGATCTGAATCCCATCAATGCTCTTAT
AATCTTGGATCAATGTCTCAGACGTCGTTTCCCACAAGACCATATCTTCATCTTCCAGAC
CGGTCTTGATCCGTACTAGATAAGTATCCTCTAATTGAACCAAAAGTCCTGTTCTTTGAC
CAAAACATCCCCACACGGTATGCTTCACTGTCTCCATACCGCTCTTGCTTCTAGATTTAA
GTCCCGAAGGCTGCGTTTCGAGCTTAAGCACAAAGCATTCTTCGTTGTTAACCGCCTTTT
CACCCACGCAAACGGATCCCGCAAATAAATTTGCTGTGGTTTTAGGATCAAGCCCTTGAA
GGAACCTTCTAAGAGGTCCAGAAGGTTCATTAGAAGCATGAGAATGTGCGAGCCAAGGGC
TTTGTCTCCAATCCACGTTACCATCACAACCAGCACTAACTTTACAACCCGAAACCACAA
GCTCTAAACTCCATTGACTTCGAA

Last Updated on 06.12.2018 16:11